02660naa a2200373 a 450000100080000000500110000800800410001910000250006024501380008526000090022352016150023265000190184765000160186665000130188265000200189565000190191565000130193465000190194765000240196665000130199065000150200365300140201865300150203265300140204765300140206170000170207570000140209270000190210670000240212570000190214970000220216870000210219077300750221120957382018-10-31       2018    bl uuuu     u00u1 u    #d1 aANDRADE-PORTO, S. M.  aHistopathological evaluation of formalin toxicity in Arapaima gigas (Arapaimidae), the giant fish from Amazon.h[electronic resource]  c2018  aThis study aimed to determine the lethal concentration and the structural and ultra-structural effects caused by the formalin exposure on juveniles of Arapaima gigas. Ninety fish (60.1± 2.5g and 20.2±0.9cm) were exposed to 0, 22, 44, 66, 88 and 110mg L-1 in order to determine the lethal concentration (LC50-96h) that was 36.4mg L-1 of formalin. Sublethal effects were evaluated using histopathological analysis on the gills and assessment of behavioral alterations and clinical signs. The LC50 of formalin for 24, 48 and 72h was 88.3, 64.7 and 56.8mg L-1 respectively. Clinical signs and behavioral changes were found: erratic swimming, lethargy, crowding on the water surface, loss of hydrodynamic equilibrium, spasms and agonistic confrontation, which were observed only at 88 and 110mg L-1. The histological alteration index (HAI) showed that 66, 88 and 100mg L-1 presented significant difference (p&lt0.05) in relation to unexposed fish, indicating that moderate damage to the gills of fish exposed to formalin had occurred. The mean values of alteration (MVA) for 22, 44, 66, 88 and 110mg L-1 were 1.14, 1.29, 1.51, 1.53 and 1.60 respectively, and differences in this index were only observed with 110 mgL-1 of formalin. It is therefore possible to conclude that sublethal concentrations of formalin (22.0mg L-1) did not compromise the health of juveniles of A. gigas. Finally, concentrations greater than to LC50-96h may be carefully used for short-term exposure, since the MVA for all concentrations tested only indicated localized lesions that did not compromise gills functionality of exposed fish.  aArapaima gigas  aArapaimidae  aFormalin  aFreshwater fish  aHistopathology  aToxicity  aHistopatologia  aPeixe de Água Doce  aPirarucu  aTratamento  aFormalina  aToxicidade  aToxicoses  aTreatment1 aRAMOS, C. A.1 aROQUE, R.1 aAFFONSO, E. G.1 aBARCELLOS, J. F. M.1 aQUEIROZ, M. N.1 aARAÚJO, C. S. O.1 aTAVARES-DIAS, M.  tPesquisa Veterinária Brasileiragv. 38, n. 6, p. 1015-1025, Jun. 2018