02246naa a2200337 a 450000100080000000500110000800800410001910000190006024501710007926000090025050001540025952010870041365000110150065000110151165000240152265000160154665000130156265000110157565000170158665300340160365300170163765300190165470000200167370000200169370000180171370000170173170000190174870000190176770000300178677300920181620948402018-08-30       2018    bl uuuu     u00u1 u    #d1 aBENASSI, J. C.  aMolecular and serological detection of Leishmania spp. in horses from an endemic area for canine visceral leishmaniasis in southeastern Brazil.h[electronic resource]  c2018  aTítulo em português: Detecção molecular e sorológica de infecção por Leishmania (Leishmania) infantum em cavalos na região Sudeste do Brasil.  aThis study aimed to verify the occurrence of Leishmania spp. and Leishmania (Leishmania) infantum in horses from a visceral leishmaniasis endemic area in Brazil. DNA samples from blood and conjunctival swab (CS) were tested by PCR and Indirect Immunofluorescence Antibody Test (IFAT). Although none of the horses was clinically sick, animals infected by Leishmania spp. were found and some could be characterized as infected by L. (L.) infantum. From 40 horses, 100% of the animals were positive by blood PCR, 90% (36/40) by CS PCR, and 2.5% (01/40) in serodiagnosis, by IFAT. Six from these 40 horses were L. (L.) infantum positive by blood PCR. Direct sequencing and analysis of amplicons resulted in a sequence to evolutionary analysis. Results indicate the presence of Leishmania spp. and L. (L.) infantum infecting healthy horses in Brazil. The presence of Leishmania spp. and L. (L.) infantum DNA in asymptomatic horses suggests that they can be important reservoirs of these parasites, a highly relevant finding for the epidemiological surveillance of the diseases they cause  aBrazil  aHorses  aLeishmania infantum  aParasitoses  aSerology  aCavalo  aLeishmaniose  aCanine visceral leishmaniasis  aEndemic area  aLeishmania spp1 aBENVENGA, G. U.1 aFERREIRA, H. L.1 aSOARES, R. M.1 aSILVA, D. T.1 aPEREIRA, V. F.1 aRUIZ, V. L. A.1 aOLIVEIR, A T. M. F. de S.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 38, n. 6, p. 1058-1063, junho 2018