01858naa a2200337 a 450000100080000000500110000800800410001910000200006024501030008026000090018350001650019252007110035765000170106865000200108565000230110565000230112865300230115165300190117465300160119365300280120965300440123765300230128165300080130470000180131270000220133070000190135270000180137170000200138970000210140977300900143020923302023-03-30       2009    bl uuuu     u00u1 u    #d1 aOBLESSUC, P. R.  aAdaptation of fluorescent technique for genotyping with new microsatellite markers in common bean.  c2009  aNotas Científicas. Título em português: Adaptação da técnica de fluorescência para fins de genotipagem com novos marcadores microssatélite em feijoeiro.  aThe objectives of this work were to adapt the fluorescent labeling polymerase chain reaction (PCR) technique using M13 universal primer for genotyping purposes, and to present a new set of microsatellite markers for common bean (Phaseolus vulgaris L.). A large population (380 common bean lines) was used for microsatellite genotyping. PCR fluorescent labeling method showed to be very efficient for multiplex analysis, providing lower costs and saving time, thus increasing the quality of genotyping analysis. A new set of 50 microsatellites developed from an enriched library derived from cultivar IAC‑UNA was presented. This study provides better tools for assisting common bean breeding programs.  aFluorescence  aGenetic markers  aMarcador Molecular  aPhaseolus Vulgaris  aAnálise multiplex  aFluorescência  aGenotipagem  aMarcação fluorescente  aMarcadores microssatélite em feijoeiro  aMultiplex analysis  aPCR1 aCAMPOS, T. de1 aCARDOSO, J. M. K.1 aSFORÇA, D. A.1 aBARONI, R. M.1 aSOUZA, A. P. de1 aBENCHIMOL, L. L.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 44, n. 6, p. 638-644, jun. 2009.