03048naa a2200409 a 450000100080000000500110000800800410001902400270006010000250008724502050011226000090031752018270032665000140215365000190216765000210218665000110220765000160221865000220223465000140225665000140227065000290228465000160231365000180232965000130234765000200236065300220238065300130240265300200241565300150243565300240245070000200247470000240249470000190251870000200253770000220255777300590257920921652018-06-04       2018    bl uuuu     u00u1 u    #d7 a10.1111/aen.123362DOI1 aISAIAS, R. M. dos S.  aFunctional compartmentalisation of nutrients and phenolics in the tissues of galls induced by Leptocybe invasa (HymenopterabEulophidae) on Eucalyptus camaldulensis (Myrtaceae).h[electronic resource]  c2018  aGalling herbivores induce structural and chemical alterations in their host plant?s tissues. These insects have been the focus of little study in the case of Australian taxa. Leptocybe invasa, a native Australian galling hymenopteran associated with Eucalyptusspecies, causes economic damage to plantation eucalypts in many countries around the world. Leptocybe invasa oviposits in the midribs and petioles of expanding leaves thereby intercepting photosynthates and impairing normal expansion. We analysed the ultrastructural and chemical cellular changes in L. invasa galls on Eucalyptus camaldulensis (probably subspecies camaldulensis) to diagnose how the insect manipulates plant cells and tissues and the significance of these alterations for insect nutrition and protection. Galling stimuli induce the formation of two functionally compartmentalised types of tissue. Phenolic plant secondary metabolites and anthocyanins (plant pigments) accumulate in the outer compartment, while primary metabolites accumulate in the inner compartment. The nutritive cells (inner compartment) accumulate protein and lipids that provide food for the larvae. Total polyphenol concentrations did not differ significantly between outer and inner compartments. Nevertheless, the concentrations of quercetin and kaempferol derivatives were higher in the outer compartment than in the inner compartment. These differences could be related to the protection of plant tissues against ultraviolet rays and the maintenance of redox homeostasis. There were higher ratios of hexahydroxydiphenoylcontaining hydrolysable tannins rather than galloyl-containing hydrolysable tannins in the inner compartment. This shift in the oxidative capacity of the polyphenols in the inner compartment could represent a defensive plant response to the larvae.  aFlavonols  aHistochemistry  aLeptocybe invasa  aLipids  aPolyphenols  aProanthocyanidins  aCitologia  aEucalipto  aEucalyptus Camaldulensis  aFlavonóide  aHistoquímica  aLipídio  aPraga de Planta  aCélula nutritiva  aCytology  aNutritive cells  aPolifenois  aTissue compartments1 aFERREIRA, B. G.1 aALVARENGA, D. R. de1 aBARBOSA, L. R.1 aSALMINEN, J.-P.1 aSTEINBAUER, M. J.  tAustral Entomologygv. 57, p. 2, p. 238-246, May 2018.