02077naa a2200277 a 450000100080000000500110000800800410001910000250006024501170008526000090020250001720021152010760038365000210145965300280148065300240150865300270153265300280155965300090158770000150159670000200161170000260163170000200165770000160167770000170169377300890171011091142018-07-30       2006    bl uuuu     u00u1 u    #d1 aNASCIMENTO, L. C. do  aEvaluation of peanut genotypes for resistance to Tomato spotted wilt virus by mechanical and thrips inoculation.  c2006  aTítulo em português: Avaliação de genótipos de amendoim em relação à resistência ao Tomato spotted wilt virus por meio de inoculação mecânica e por tripes.  aThe objective of this work was to evaluate the reactions of three peanut breeding lines (IC-10, IC-34, and ICGV 86388) to Tomato spotted wilt virus (TSWV) by mechanical and thrips inoculation, under greenhouse conditions, and compare them to the reactions of cultivars SunOleic, Georgia Green, and the breeding line C11-2-39. TSWV infection by mechanical inoculation was visually assessed using an index ranging from 0 (no symptoms) to 4 (apical death). Enzyme-linked immunosorbent assay was used to confirm TSWV infection from both mechanical and thrips inoculations. IC-10, IC-34, ICGV 86388, and C11-2-39 were more resistant than the cultivars SunOleic and Georgia Green based on mechanical inoculation. Upon thrips inoculation only IC-34 and ICGV-86388 were infected by TSWV, as demonstrated by reverse transcription polymerase chain reaction (RT-PCR), although no symptoms of infection were observed. The peanut breeding lines IC-10, IC-34, and ICGV 86388 show higher level of resistance to TSWV than cultivar Georgia Green considered a standard for TSWV resistance.  aArachis Hypogaea  amechanical transmission  athrips transmission  atransmissão mecânica  atransmissão por tripes  aTSWV1 aPENSUK, V.1 aCOSTA, N. P. da1 aASSIS FILHO, F. M. de1 aPIO-RIBEIRO, G.1 aDEOM, C. M.1 aSHERWOOD, J.  tPesquisa Agropecuária Brasileira, Brasília, DFgv. 41, n. 6, p. 937-942, jun. 2006