02954naa a2200337 a 450000100080000000500110000800800410001910000260006024501700008626000090025650001630026552017660042865000300219465000200222465000110224465000110225565000190226665000100228565000140229565000110230965300360232065300250235670000170238170000160239870000180241470000250243270000240245770000190248170000190250077300970251920893482018-03-19       2017    bl uuuu     u00u1 u    #d1 aNOQUEIRA, R. de M. S.  aMolecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil.h[electronic resource]  c2017  aTítulo em português: Detecção molecular e serológica de Theileria equi, Babesia caballi e Anaplasma phagocytophilum em equinos e carrapatos no Maranhão.  aEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p&gt0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.  aAnaplasma phagocytophilum  aBabesia caballi  aBrazil  aHorses  aTheileria equi  aTicks  aCarrapato  aCavalo  aDoença transmitida por vetores  aVector-borne disease1 aSILVA, A. B.1 aSATO, T. P.1 aSÁ, J. C. de1 aSANTOS, A. C. G. dos1 aAMORIM FILHO, E. F.1 aVALE, T. L. do1 aGAZÊTA, G. S.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 37, n. 12, p. 1416-1422, dezembro 2017.