02034naa a2200361 a 450000100080000000500110000800800410001910000190006024501400007926000090021952009200022865000190114865000100116765300250117765300240120265300240122665300300125065300190128065300280129965300300132765300160135765300180137370000220139170000190141370000220143270000210145470000200147570000170149570000320151270000210154470000190156577300880158420877962018-05-15 2017 bl uuuu u00u1 u #d1 aLIMA, H. L. S. aBacterial cellulose production by Komagataeibacter hansenii ATCC 23769 using sisal juiceban agroindustry waste.h[electronic resource] c2017 aIn this study, we have optimized production of bacterial cellulose (BC) by Komagataeibacter hansenii ATCC 23769 in a static cultivation using sisal juice, an agroindustrial residue, as substrate. Optimization of fermentation parameters has been carried out using the one-variable-at-a-time method. Effect of initial sugar concentration, pH, nitrogen supplement, and cultivation time was evaluated. The influence of nitrogen source and quantity for bacterial cellulose production was studied using a central composite rotational design (CCRD).The highest production of BC (3.38 g/L) was obtained after 10 days of cultivation, using sisal juice (pH 5) at 15 g/L of sugars and supplemented with 7.5 g/L of extract yeast. The cellulose production yield in selected sisal culture conditions was three times higher than the yield in synthetic medium, indicating that sisal juice is a suitable substrate for BC production. aNanotechnology aSisal aAgroindustrial waste aBacterial cellulose aCelulose bacteriana aKomagataeibacter hansenii aNanotecnologia aResíduo agroindustrial aResíduos Agroindustriais aSisal juice aSuco de sisal1 aNASCIMENTO, E. S.1 aANDRADE, F. K.1 aBRIGIDA, A. I. S.1 aBORGES, M. de F.1 aCASSALES, A. R.1 aMUNIZ, C. R.1 aSOUZA FILHO, M. de S. M. de1 aMORAIS, J. P. S.1 aROSA, M. de F. tBrazilian Journal of Chemical Engineeringgv. 34, n. 3, p. 671-680, Jul./Sep. 2017.