01590naa a2200313 a 450000100080000000500110000800800410001902400320006010000200009224501520011226000090026452006600027365000270093365000150096065300160097565300180099165300230100965300180103270000180105070000170106870000190108570000140110470000170111870000190113570000170115470000190117170000160119077300700120620845132018-01-10 2017 bl uuuu u00u1 u #d7 adoi:10.1093/jxb/erx1662DOI1 aALVES, G. S. C. aDifferential fine-tuning of gene expression regulation in coffee leaves by CcDREB1D promoter haplotypes under water deficit.h[electronic resource] c2017 aDespite the importance of the DREB1D gene (also known as CBF4) in plant responses to water deficit and cold stress, studies analysing its regulation by transgenic approaches are lacking. In the current work, a functional study of three CcDREB1D promoter haplotypes (named HP15, HP16 and HP17) isolated from drought-tolerant and droughtsensitive clones of Coffea canephora was carried out in plants of C. arabica stably transformed by Agrobacterium tumefaciens by analysing their ability to regulate the expression of the uidA reporter gene in response to water deficit mimicked by polyethylene glycol (−2.0 MPa) and low relative humidity treatments. aGenetic transformation aHaplotypes aDREB1D gene aReporter gene aUidA reporter gene aWater deficit1 aTORRES, L. F.1 aDÉCHAMP, E.1 aBREITLER, J-C.1 aJOËT, T.1 aGATINEAU, F.1 aANDRADE, A. C.1 aBERTRAND, B.1 aMARRACCINI, P.1 aETIENNE, H. tJournal of Experimental Botanygv. 68, n. 11, p. 3017-3031, 2017.