02474naa a2200253 a 450000100080000000500110000800800410001910000180006024501540007826000090023252016850024165000230192665000180194965300270196765300160199470000190201070000280202970000200205770000200207770000220209770000170211970000190213677300650215520826882017-12-15       2017    bl uuuu     u00u1 u    #d1 aFLORES, J. C.  aHigh throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection.h[electronic resource]  c2017  aCoffee rust disease, caused by the fungus Hemileia vastatrix, is one of the major diseases in coffee throughout the world. The use of resistant cultivars is considered to be the most effective control strategy for this disease. To identify candidate genes related to different mechanism defense in coffee, we present a time-course comparative gene expression profile of Caturra (susceptible) and Híbrido de Timor (HdT, resistant) in response to H. vastatrix race XXXIII infection. The main objectives were to obtain a global overview of transcriptome in both interaction, compatible and incompatible, and, specially, analyze up-regulated HdT specific genes with inducible resistant and defense signaling pathways. Using both Coffea canephora as a reference genome and de novo assembly, we obtained 43,159 transcripts. At early infection events (12 and 24 h after infection), HdT responded to the attack of H. vastatrix with a larger number of up-regulated genes than Caturra, which was related to prehaustorial resistance. The genes found in HdT at early hours were involved in receptor-like kinases, response ion fluxes, production of reactive oxygen species, protein phosphorylation, ethylene biosynthesis and callose deposition. We selected 13 up-regulated HdTexclusive genes to validate by real-time qPCR, which most of them confirmed their higher expression in HdT than in Caturra at early stage of infection. These genes have the potential to assist the development of new coffee rust control strategies. Collectively, our results provide understanding of expression profiles in coffee?H. vastatrix interaction over a time course in susceptible and resistant coffee plants.  aDisease resistance  aTranscriptome  aBiotrophic interaction  aCoffee rust1 aMOFATTO, L. S.1 aFREITAS-LOPES, R. do L.1 aFERREIRA, S. S.1 aZAMBOLIM, E. M.1 aCARAZZOLLE, M. F.1 aZAMBOLIM, L.1 aCAIXETA, E. T.  tPlant Molecular Biologygv. 95, n. 6, p. 607-623, Dec. 2017.