02976naa a2200385 a 450000100080000000500110000800800410001902400530006010000180011324501530013126000090028452018080029365000150210165000140211665000110213065000180214165000140215965000240217365300190219765300190221665300130223570000150224870000230226370000260228670000200231270000250233270000230235770000150238070000190239570000190241470000200243370000200245370000230247377300940249620819392018-03-09       2017    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1007/s11274-017-2307-52DOI1 aOTTONI, J. R.  aFunctional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest.h[electronic resource]  c2017  aMangroves are located in coastal wetlands and are susceptible to the consequences of oil spills, what may threaten the diversity of microorganisms responsible for the nutrient cycling and the consequent ecosystem functioning. Previous reports show that high concentration of oil favors the incidence of epoxide hydrolases and haloalkane dehal- ogenases in mangroves. This finding has guided the goals of this study in an attempt to broaden the analysis to other hydrolases and thereby verify whether oil contamination interferes with the prevalence of particular hydrolases and their assigned microorganisms. For this, an in-depth survey of the taxonomic and functional microbial diversity recov- ered in a fosmid library (Library_Oil Mgv) constructed from oil-impacted Brazilian mangrove sediment was car- ried out. Fosmid DNA of the whole library was extracted and submitted to Illumina HiSeq sequencing. The resulting Library Oil_Mgv dataset was further compared with those obtained by direct sequencing of environmental DNA from Brazilian mangroves (from distinct regions and affected by distinct sources of contamination), focusing on hydro- lases with potential use in biotechnological processes. The most abundant hydrolases found were proteases, esterases and amylases, with similar occurrence profile in all data- sets. The main microbial groups harboring such hydrolase- encoding genes were distinct in each mangrove, and in the fosmid library these enzymes were mainly assigned to Chloroflexaceae (for amylases), Planctomycetaceae (for esterases) and Bradyrhizobiaceae (for proteases). Assem- bly and analysis of Library_Oil Mgv reads revealed three potentially novel enzymes, one epoxide hydrolase, one xylanase and one amylase, to be further investigated via heterologous expression assays.  aHydrolases  aHidrolase  aMangue  aMicrorganismo  aPetróleo  aPoluição da água  aBioprospecting  aFosmid library  aMangrove1 aCABRAL, L.1 aSOUSA, S. T. P. de1 aLACERDA JUNIOR, G. V.1 aDOMINGOS, D. F.1 aSOARES JUNIOR, F. L.1 aSILVA, M. C. P. da1 aMARCON, J.1 aDIAS, A. C. F.1 aMELO, I. S. de1 aSOUZA, A. P. de1 aANDREOTE, F. D.1 aOLIVEIRA, V. M. de  tWorld Journal of Microbiology and Biotechnologygv. 33, n. 7, p. 1-13, 2017. Article 141.