02151naa a2200289 a 450000100080000000500110000800800410001902400560006010000170011624501340013326000090026752012780027665000190155465000190157365000220159265000230161465000240163765000120166165300140167365300220168765300140170970000270172370000160175070000170176670000230178377300550180620735782017-10-23       2017    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1016/j.envpol.2017.03.0332DOI1 aCLEMENTE, Z.  aNanotoxicity of graphene oxidebassessing the influence of oxidation debris in the presence of humic acid.h[electronic resource]  c2017  aAbstract: This study sought to evaluate the toxicological effects of graphene oxide (GO) through tests with Danio rerio (zebrafish) embryos, considering the influence of the base washing treatment and the interaction with natural organic matter (i.e., humic acid, HA). A commercial sample of GO was refluxed with NaOH to remove oxidation debris (OD) byproducts, which resulted in a base washed GO sample (bw-GO). This process decreased the total oxygenated groups in bw-GO and its stability in water compared to GO. When tested in the presence of HA, both GO and bw-GO stabilities were enhanced in water. Although the embryo exposure showed no acute toxicity or malformation, the larvae exposed to GO showed a reduction in their overall length and acetylcholinesterase activity. In the presence of HA, GO also inhibited acid phosphatase activity. Our findings indicate a mitigation of material toxicity after OD removal. The difference in the biological effects may be related to the materials? bioavailability and biophysicochemical interactions. This study reports for the first time the critical influence of OD on the GO material biological reactivity and HA interaction, providing new data for nanomaterial environmental risk assessment and sustainable nanotechnology.  aembryotoxicity  ananotechnology  aComposto químico  aMatéria orgânica  aPeixe de água doce  aToxidez  aBiomarker  aNanoecotoxicology  aZebrafish1 aCASTRO, V. L. S. S. de1 aFRANQUI, L.1 aSILVA, C. A.1 aMARTINEZ, D. S. T.  tEnvironmental Pollutiongv. 225, p. 118-128, 2017.