01663naa a2200205 a 450000100080000000500110000800800410001902400470006010000220010724501410012926000090027052010040027965000140128365300080129765300140130570000250131970000170134470000190136177300770138020724382024-04-09       2017    bl uuuu     u00u1 u    #d7 ahttps://doi.org/10.1038/ejcn.2017.1072DOI1 aGAMA, M. A. S. da  aAnalytical shortcomings and other considerations related to the identification of biomarkers of dairy fat intake.h[electronic resource]  c2017  aOver the last decade, the concentrations of certain odd-chain saturated fatty acids (for example, C15:0 and C17:0) and trans-palmitoleic acid (trans-9 C16:1) in plasma and erythrocytes have been proposed as biomarkers of dairy fat intake,1, 2, 3 which is expected to overcome major issues associated with dietary assessment methods that rely on self-report, such as food frequency questionnaires. However, some overlooked analytical issues may have resulted in misidentification or even precluded other candidate biomarkers of dairy fat intake from being identified in several previous studies. The separation of fatty acids in biological and foods matrices is usually performed by gas chromatography after lipid extraction and methylation/transesterification using either acid or alkali-catalysis. Fatty acid methyl esters (FAME) are routinely identified by retention time comparisons with authentic commercial standards using a gas chromatograph equipped with a flame ionization detector (GC-FID).  anutrition  aCLA  aFat acids1 aBARBOSA FILHO, H. G.1 aBIZZO, H. R.1 aANTONIASSI, R.  tEuropean Journal of Clinical Nutritiongv. 71, n. 8, p. 1022-1023, 2017.