01704nam a2200217 a 450000100080000000500110000800800410001910000220006024501290008226001240021152009510033565300280128670000240131470000260133870000190136470000230138370000250140670000190143170000190145070000170146920661992023-03-29       2016    bl uuuu     u01u1 u    #d1 aSANTOS, R. C. dos  aInitial assessment of GM cotton resistant to cotton boll weevil based on feeding bioassays and Elisa.h[electronic resource]  aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPEc2016  aThe boll weevil (Anthonomus grandis) is the main pest of cotton crop due to cause serious damages to reproductive structures, affecting directly the yield. The effective control is done through chemical insecticides, which substantially increase management costs. Control via transgene sis is a promising strategy and less harmful to the environment, especially by using Cry proteins, derived from endophytic Bt bacteria. In 2015, the biotech team from Embrapa introduced a cry 10-construction into cotton plants, isolated from a Bt strain that showed low DL50 in boll weevil feeding bioassays (7.12 mg/mL). In order to test the toxicity of Cry proteins were conducted in laboratory bioassays feeding with boll weevil and ELISA assays. Three hundred and sixty plants T0 were grown in green house and further were evaluated in feeding bioassays and ELISA tests, carried out in Entomology and Biotech Labs, at Embrapa Algodão (Campina Grande, PB).  aMelhoramento de plantas1 aDUARTE, M. de M. F.1 aSILVA, M. DE F. C. DA1 aBRAZ, L. C. C.1 aSILVA, C. R. C. DA1 aCAVALCANTI, J. J. V.1 aLIMA, L. M. de1 aMARTINS, E. S.1 aMONNERAT, R.