01499naa a2200265 a 450000100080000000500110000800800410001910000230006024501820008326000090026552006290027465300340090365300240093765300230096170000250098470000200100970000240102970000200105370000180107370000200109170000220111170000230113370000200115677300570117620660502017-03-03       2016    bl uuuu     u00u1 u    #d1 aOLIVEIRA, R. S. de  aTransgenic cotton plants expressing Cry1la12 toxin confer resistance to fall armyworm (Spodoptera frugiperda) and cotton boll weevil (Anthonomus grandis).h[electronic resource]  c2016  aGossypium hirsutum (commercial cooton) is one of the most economically important fibers sources and a commodity crop highly affected by insect pests and pathogens. Several transgenic approaches have been developed to improve cotton resistance to insect pests, through the transgenic expression of different factors, including Cry toxins, proteinase inhibitors, and toxic peptides, among others. In the present study, we developed transgenic cotton plants by fertilized floral buds injection (through the pollen-tube pathway technique) using an DNA expression cassette harboring the cry1Ia12 gene, driven by CaMV35S promoter.  aGenetic cotton transformation  aGosspypium hirsutum  aPolen-tube pathway1 aOLIVEIRA NETO, O. B.1 aMOURA, H. F. N.1 aMACEDO, L. L. P. de1 aARRAES, F. B M.1 aLUCENA, W. A.1 aLOURENCO, I. T.1 aBARBOSA, A. de D.1 aSILVA, M. C. M. da1 aSA, M. F. G. de  tFrontiers in Plant Sciencegv. 7, Article 165, 2016.