02162naa a2200277 a 450000100080000000500110000800800410001910000180006024501880007826000090026652012690027565000160154465000090156065000140156965000180158365000360160165300090163765300300164670000190167670000170169570000170171270000210172970000200175070000210177077300930179120643142017-12-29 2016 bl uuuu u00u1 u #d1 aBOTONI, L. S. aPrevalence and in vitro susceptibility of methicillin-resistant Staphylococcus pseudintermedius (MRSP) from skin and nostrils of dogs with superficial pyoderma.h[electronic resource] c2016 aIn order to assess the prevalence of Methicillin-resistant Staphylococcus pseudintermedius from skin and nostrils of dogs with pyoderma, to determine its in vitro susceptibility, and to correlate these data with the presence of the mecA gene, 43 dogs were selected. Samples were collected from secretion of their skin lesions and right nostril, cultured, and analyzed for phenotypic and genotypic characteristics of the bacteria studied. In 62 samples (91%) the microorganism was classified as S. pseudintermedius. The rate of resistance against antibiotics ranged from 7% (amikacin; 4/62) to 77% (sulfamethoxazole + trimethoprim; 48/62). Resistance against oxacillin was found in 34% of the samples (21/62). Twenty-five samples (37%) were strains that carried the mecA gene. A significant correlation (P<0.01) was found between presence of the mecA gene and oxacillin resistance. Seventeen dogs were mecA gene carriers, and 8 (47%) of them had the gene in the skin lesions and nostril. A significant correlation (P<0.01) was also observed between the presence of mecA gene in the skin lesions and nostrils. Oxacillin resistance in vitro can be safely used to indicate the presence of mecA gene in MRSP samples. The nostrils can be a reservoir of MRSP in dogs. aAntibiotics aDogs aOxacillin aSkin diseases aStaphylococcus pseudintermedius aMRSP aStaphylococcal infections1 aSCHERER, C. B.1 aSILVA, R. O.1 aCOURA, F. M.1 aHEINEMANN, M. B.1 aPAES-LEME, F. O1 aCOSTA-VAL, A. P. tPesquisa VeterinĂ¡ria Brasileira, Rio de Janeirogv. 36, n. 12, p. 1178-1180, dez. 2016.