01445naa a2200241 a 450000100080000000500110000800800410001910000150006024501370007526000090021252007160022165000200093765000220095765000180097965000190099765000100101665300290102670000170105570000180107270000200109070000140111077300790112420617532017-01-25       1980    bl uuuu     u00u1 u    #d1 aSARKAR, P.  aApplication of fluorescent antibody test in the diagnosis of sheep-pox, and study of sheep-pox virus multiplication in cell-culture.  c1980  aAbstract : Direct fluorescent antibody test (FAT) was used to detect the sheep pox virus (SPV) in the oedema fluid of infected sheep, with or without traces of tissue fibres. Bright apple-green fluorescence against a dark-grey background was observed under the fluorescence microscope. The virus multiplied in monolayer cultures of lamb kidney cells. Depending upon the aggregation of virus particles, the size and brightness of the fluoresced masses became gradually prominent, and covered considerable areas of the cell cytoplasm. By indirect FAT, antibodies against SPV were detected with precision by adopting a two-stage staining procedure. This method can be used to detect the antibody status of a flock.  aAnimal diseases  aAnimal production  aCapripoxvirus  aDoença animal  aOvino  aVírus da varíola ovina1 aSINGH, S. P.1 aPANDEY, A. K.1 aKATHURIA, B. K.1 aKUMAR, S.  tThe Indian Journal of Animal Sciencesgv. 50, n. 5, p. 428-433, May. 1980.