01686naa a2200205 a 450000100080000000500110000800800410001902200140006010000130007424501170008726000090020452011110021365000210132465000220134570000200136770000210138770000150140870000200142377300370144320616632017-12-04       2017    bl uuuu     u00u1 u    #d  a1300-01521 aKAYA, E.  aCryopreservation of citrus seed via dehydration followed by immersion in liquid nitrogen.h[electronic resource]  c2017  aAn important method for plant germplasm conservation is offered by a biotechnology-based approach of cryopreservation. Cryopreservation refers to the storage of plant material at ultralow temperatures in liquid nitrogen. A procedure for cryopreservation of polyembryonic seeds was improved for selected citrus cultivars from Turkey. Seed dehydration was performed at different exposure times, in sterile conditions of a laminar flow-hood. The tested cultivars showed the highest tolerance to low temperature storage when the seeds were first dehydrated to a moisture content of 21.8% for Poncirus trifoliata Raf. × Citrus sinensis Osb. and to 17.6% for Citrus limonia Osbeck. The postcryopreservation germinability ranged from 73.3% (Poncirus trifoliata Raf. × C. sinensis Osb. and Fortunella margarita (Lour.) Swingle) to 93.3% (C. jambhiri Lush.). Dehydration was beneficial for germination of seeds from all of the tested citrus species after the liquid nitrogen exposure. Seedlings derived from cryopreserved seeds had well-formed shoots and roots and were easily acclimated to greenhouse conditions.  aCryopreservation  aCriopreservação1 aSOUZA, F. V. D.1 aGÖKDOGAN, E. Y.1 aCEYLAN, M.1 aJENDEREK, M. M.  tTurkish Journal of Biology, 2017