02082naa a2200217 a 450000100080000000500110000800800410001902200130006010000220007324501460009526000090024152013960025065000190164665300110166570000250167670000170170170000250171870000220174370000260176577300730179120614342018-01-26       2016    bl uuuu     u00u1 u    #d  a167656801 aSANTANA, J. G. S.  aEstimation of genetic diversity in a natural population of cambui tree (Myrciaria tenella O. Berg) using ISSR markers.h[electronic resource]  c2016  aCambui (Myrciaria tenella O. Berg) is a native species from  Brazil, which belongs to the family Myrtaceae. Molecular characterization is one of the most used tools for the study of the biotechnological potential of species because the diversity level between individuals can be inferred. Analysis of  enetic diversity is fundamental to the direction of the strategies necessary to form and maintain a germplasm. This study aimed to evaluate the genetic diversity in a natural population of cambui using inter-simple  sequence repeat (ISSR) molecular markers. The natural population, which provided the plant material, is found at the Private Reserve of Natural Heritage of Caju, which belongs to the experimental field of Embrapa Tabuleiros Costeiros, in the municipality of Itaporanga d’Ajuda, SE, Brazil. Young leaves of each individual were collected for DNA extraction and analysis of PCR-ISSR. Thirty primers were tested and the top 10 were selected. The use of these primers resulted in 71 fragments with 98.3%  polymorphism. Similarity of individuals ranged  between 0.30 and 0.92. The most similar individuals were C13 and C17 and the most distant were C1 and C41. Through UPGMA, six distinct groups were identified. This information may be used for conservation of these genetic  resources, germplasm  exchange, creation of germplasm bank and in future studies with this species.  aFruta tropical  aCambui1 aNASCIMENTO, A. L. S.1 aCOSTA, T. S.1 aALMEIDA, T. M. B. de1 aRABBANI, A. R. C.1 aMUNIZ, A. V. C. da S.  tGenetics and Molecular Research, Ribeirão Pretogv. 15, n. 4, 2016.