01800naa a2200265 a 450000100080000000500110000800800410001902400580006010000240011824501410014226000090028352009860029265000240127865000200130265000120132265000110133465300260134570000190137170000230139070000130141370000180142670000200144470000180146477300520148220583602019-07-18       2016    bl uuuu     u00u1 u    #d7 ahttp://dx.doi.org/10.1016/j.biortech.2016.07.0312DOI1 aSTEINMETZ, R. L. R.  aEnrichment and acclimation of an anaerobic mesophilic microorganism?s inoculum for standardization of BMP assays.h[electronic resource]  c2016  aAppropriate enrichment of anaerobic microorganism?s consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857 days in order to reach steady conditions (pH = 7.90 ± 0.46; VS/TS &gt 50%; VFA/alkalinity = 0.16 ± 0.04 gAcetic Acid/gCaCO3 ). Biogas yield &gt80% was obtained after 70 days of inoculum acclimation in comparison to standard cellulose (&gt600 mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3 1012 gene copies/g (Methanobacteriales 8 1010; Methanomicrobiales 8 1010; and Methanosarcinales 4 1011 gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations.  aAnaerobic digestion  aWaste treatment  aBiogás  aDejeto  aDigestão anaeróbica1 aMEZZARI, M. P.1 aSILVA, M. L. B. da1 aKUNZ, A.1 aAMARAL, A. C.1 aTÁPPARO, D. C.1 aSOARES, H. M.  tBioresource Technologygv. 219, p. 21-28, 2016.