03031naa a2200277 a 450000100080000000500110000800800410001910000170006024501320007726000090020952022690021865000240248765000090251165000140252065000090253465000140254365000180255765000130257565000220258870000180261070000140262870000170264270000190265970000160267877300590269420570662016-11-24       2016    bl uuuu     u00u1 u    #d1 aPUGLIESI, G.  aImproved fertility in suckled beef cows ovulating large follicles or supplemented with long-acting progesterone after timed-AI.  c2016  aWe aimed to evaluate the effects and the interaction of size of the preovulatory follicle (POF) and long-acting progesterone (P4) supplementation after timed-AI on CL function and pregnancy success in beef cows. In experiment 1, ovulations of beef cows were synchronized starting on Day 10, and cows were split to receive sodium cloprostenol (large follicle group; LF; n ¼ 31) or nothing (small follicle group; SF; n ¼ 35). Ovulations were induced on Day 0, and cows were inseminated. Ovulated cows were assigned to receive placebo (LF/control group, n ¼ 14; and SF/control group, n ¼ 9) or 150 mg of long-acting P4 on Day 4.5 (LF/P4 group, n ¼ 13; and SF/P4 group, n ¼ 12). Diameter of POF, blood flow in POF wall, ovulation rate, and size and vascularization of CL were greater (P &lt 0.05) in LF group. In experiments 2 (unknown cyclic status) and 4 (noncycling), ovulations were synchronized, and beef cows received placebo or 150 mg of long-acting P4 on Day 4 after timed-artificial insemination. In experiment 2, pregnancy/AI (P/AI) did not differ (P &gt 0.1) between P4-treated (53.2%; 209/393) and control cows (56.2%; 219/390), but P/AI was greater in cows with a CL &lt 0.9 cm2 on Day 4 that were P4-treated (57.9%, 22/38) versus placebo-treated (40.4%, 21/52; P &lt 0.05). In Experiment 4, P/AI was greater (P &lt 0.05) in P4-treated cows (55.6%, 105/189 vs. 46.0%, 86/187). In Experiment 3, cyclic?suckled beef cows were treated as described in Experiment 1 to generate animals with small (SF; n ¼ 111) or large POF (LF; n ¼ 109), and subdivided to receive placebo or P4 on Day 4. POF size, ovulation rate, CL area, and P/AI were greater (P &lt 0.007) in the LF group. Pregnancy/AI in ovulated cows were lower (P ¼ 0.05) in the SF/control group (41.5%, 17/41) compared to LF/control group (62%, 31/50) and were similar for the SF/P4 group (55.6%, 25/45) and LF/P4 group (57%, 28/49) compared to others. In summary, smaller and less vascularized POF results in less functional CL and reduces ovulatory rate and P/AI in cyclic beef cows; the long-acting P4 injection on Day 4 after timed-artificial insemination may attenuate the negative effects of small POF/CL; and postovulatory P4 supplementation improved fertility in anestrous beef cattle.  aAnimal reproduction  abeef  aBeef cows  acows  aOvulation  aGado de corte  aOvulacao  aReproducao animal1 aSANTOS, F. B.1 aLOPES, E.1 aNOGUEIRA, E.1 aMAIO, J. R. G.1 aBINELLI, M.  tTheriogenologygn. 85, n. 7, p. 1239-1248, April 2016.