01398naa a2200229 a 450000100080000000500110000800800410001910000150006024501120007526000090018752007250019665000190092165300200094065300280096065300220098865300080101065300110101870000160102970000220104570000200106777300810108711054772004-09-27 1993 bl uuuu u00u1 u #d1 aMANSUR, E. aIn Vitro Regeneration of Arachis Villosulicarpa Hoehne from Cotyledon Segments, Leaves and Cell Suspension. c1993 aIn vitro regeneration from immature leaves and cotyledon segments of A. villosuli-carpa Hoehne was obtained on Murashige & Skoog medium (1962) plus 1.0 mg/l 1-naphthalene acetic acid (NAA) and 1.0 mg/l 6-benzylaminopurine (BAP). Cotyledon segments showed higher morphogenetic potential than immature leaves. Morphogenic calli could also be obtained from leaves of regenerated plants propagated in vitro. Cell suspension cultures were established from friable calli originated from leaf and cotyledon explants cultured on MS medium supplemented with 0.2 mg/l picloram and 1.0 mg/l BAR. Calli from liquid culture developed shoots on regenera-tion medium. Root differentiation of plantlets was achieved on liquid MS medium. atissue culture aImmature leaves amorphogenetic potential amorphogenic calli aNAA apeanut1 aLACORTE, C.1 aRABELLO, A. C. G.1 aCORDEIRO, A. R. tPesquisa Agropecuaria Brasileira, Brasiliagv.28, n.10, p.1143-1146,out.1993