01985naa a2200277 a 450000100080000000500110000800800410001910000180006024500940007826000090017252012350018165000150141665000180143165000130144965000220146265000200148465000170150470000170152170000160153870000260155470000220158070000190160270000210162170000180164277300470166020529052017-07-14       2016    bl ---       0--  u    #d1 aVIEIRA, A. S.  aDetection of wild animals as carriers of Leptospira by PCR in the Pantanal biome, Brazil.  c2016  aLeptospiral infection is widespread in wildlife. In this context, wild ecosystems in tropical countries hold a vast biodiversity, including several species thatmay act as potential reservoirs ofleptospires. The Pantanal biome presents highly favorable environmental conditions for the occurrence of leptospirosis, such as high temperatures, constant flooding, and high biodiversity. The purpose of this study was to detect wild animals as carriers of Leptospira sp. using direct methods (PCR and culture) in the Pantanal biome, Brazil. A total of 35 animals were studied, namely Cerdocyon thous, Nasua nasua, Ozotoceros bezoarticus, and Sus scrofa species. Blood for serology (MAT) and urine for bacteriological culturing and PCR was sampled. The most prevalent serogroups were Javanica and Djasiman. Additionally, 40.6% of these animals presented PCR positive reactions. Seroreactivity associated with the high frequency ofleptospiral carriers among the different studied species suggests a high level of exposure of the studied animals to pathogenic Leptospira strains. Our results are still limited and the actual role of the studied animals in the epidemiology of leptospirosis in the Pantanal region remains to be elucidated.  aLeptospira  aleptospirosis  awildlife  aWildlife diseases  aAnimal selvagem  aLeptospirose1 aNARDUCHE, L.1 aMARTINS, G.1 aPERES, I. A. H. F. S.1 aZIMMERMANN, N. P.1 aJULIANO, R. S.1 aPELLEGRIN, A. O.1 aLILENBAUM, W.  tActa Tropicagv. 163, p. 87-89, aug. 2016.