02012naa a2200217 a 450000100080000000500110000800800410001910000190006024501590007926000090023852013160024765000120156365300310157565300170160670000250162370000250164870000180167370000190169170000290171077300550173920518172022-05-24       2016    bl uuuu     u00u1 u    #d1 aPEREIRA, S. G.  aPhytotoxic potential of piperine and extracts from fruits of Piper tuberculatum Jaq. on Senna obtusifolia and Mimosa pudica plants.h[electronic resource]  c2016  aWe evaluated the in vitro efficacy of fruits extracts of Piper tuberculatum Jaq. on lhe gennination and growth of Senna obtusifolia and Mimosa pudica and also the phytotoxicity of main compound, piperine. The extracts 01' hexane (HE), ethyl ether (EE), methanol (EM) and hot ethanol (EEq) completely inhibited the germination of M. pudica at 5.0 mg ml,' concentration and only EE caused 62% inhibition in gennination of S. obtusifolia at this concentration. Jn chemical investigation using GC-MS of samples of the extracts EE, EM and EEq, it was found that the concentration of piperine in these extracts was 33.65%, 4533% and 41.88%, respectively. The column chromatography for isolation and purification confinned that piperine was the key compound in EE, EM and EEq extracts. Bioassays with piperine at 0.06, 0.12, 0.25, 0.50 and 100 mg rnl,' concentrations showed significant phytotoxicity to seeds germination and seedling growth 01'both test plants. The mean inhibitory concentration (ICso) for S obtusifolia hypocolyl was 0.86 and 0.98 mg ml." for radicle and the M. pudica ICso values were 0.51 mg ml.' for radicle and 0.53 mg ml,' for hypocotyl, respectively. This research showed that fruits 01' P. tuberculatum plant and the isolated substances were phytotoxic and showed high allelopathic potential.  aPimenta  aCrescimento das plântulas  aFitotoxidade1 aSOARES, A. M. dos S.1 aGUILHON, G. M. S. P.1 aSANTOS, L. S.1 aPACHECO, L. C.1 aSOUZA FILHO, A. P. da S.  tAllelopathy Journalgv. 38, n. 1, p. 91-102, 2016.