02432naa a2200337 a 450000100080000000500110000800800410001902200140006002400530007410000240012724001330015124501500028426000090043452012710044365000140171465000140172865000130174265300140175565300270176965300130179665300250180965300090183470000160184370000180185970000220187770000220189970000240192170000200194570000250196577301040199020484612022-05-23       2016    bl uuuu     u00u1 u    #d  a0103-846X7 ahttp://dx.doi.org/10.1590/S1984-296120160322DOI1 aCASTRO, K. N. de C.  aEfeitos in vitro do extrato de Pilocarpus microphyllus e do cloridrato de pilocarpina sobre Rhipicephalus (Boophilus) microplus.  aIn vitro effects of Pilocarpus microphyllus extracts and pilocarpine hydrochloride on Rhipicephalus (Boophilus) microplus.h[electronic resource]  c2016  aThe aim of this study was to assess the activity of aqueous (AE) and ethanolic extracts (EE) and pilocarpine hydrochloride, which were extracted and isolated from Pilocarpus microphyllus (Jaborandi), respectively, on Rhipicephalus (Boophilus) microplus. High performance liquid chromatography (HPLC) was performed to quantify these compounds. Larval packet and adult immersion tests were conducted with different concentrations. Five AE and EE concentrations, ranging from 6.2 to 100.0 mg mL?1, and six concentrations of pilocarpine hydrochloride, ranging from 0.7 to 24.0 mg mL?1, were tested. The lethal concentration (LC50) of each extract for larvae and engorged females was calculated through Probit analysis. The concentration of pilocarpine hydrochloride obtained from the EE and the AE was 1.3 and 0.3% (m/m), respectively. Pilocarpine hydrochloride presented the highest acaricidal activity on larvae (LC50 2.6 mg mL?1) and engorged females (LC50 11.8 mg mL?1) of R.(B.) microplus, followed by the EE which presented LC50 of 56.4 and 15.9 mg mL?1, for larvae and engorged females, respectively. Such results indicate that pilocarpine hydrochloride has acaricidal activity, and may be the primary compound responsible for this activity by P. microphyllus EE.  aAcaricida  aCarrapato  aParasito  aAcaricide  aControle fitoterápico  aParasite  aPhytotherapy control  aTick1 aLIMA, D. F.1 aWOLSCHICK, D.1 aANDRADE, I. M. de1 aSANTOS, R. C. dos1 aSANTOS, F. J. de S.1 aVERAS, L. M. C.1 aCOSTA-JÚNIOR, L. M.  tBrazilian Journal of Veterinary Parasitology, Jaboticabalgv. 25, n. 2, p. 248-253, abr./jun. 2016.