01651naa a2200289 a 450000100080000000500110000800800410001910000150006024501010007526000090017652008050018565000160099065000270100665000150103365000180104865300240106665300190109065300220110970000200113170000200115170000260117170000190119770000210121670000150123770000190125277300900127120424712018-08-01       2016    bl uuuu     u00u1 u    #d1 aBRANCO, E.  aProtocol for collection and deparation of bone marrow mononuclear cells in Chlorocebus aethiops.  c2016  aChlorocebus aethiops is a species of non-human primate frequently used in biomedical research. Some research involves this species as an experimental model for various diseases and possible treatment with stem cells. The bone marrow is one of the main sources of these cells and provides easy access. The aim of this study was to standardize the protocol of collection and separation of bone marrow in C. aethiops. Ten animals were submitted to puncture of bone marrow with access to the iliac crest and cell separation by density gradient. The bone marrow of C. aethiops had an average of 97% viability. From the results achieved, we can conclude that C. aethiops is an excellent model to obtain and isolate mononuclear cells from bone marrow, fostering several studies in the field of cell therapy.  abone marrow  aCercopithecus aethiops  aStem cells  aMedula óssea  aCélula mononuclear  aCélula-tronco  aMononuclear cells1 aSILVA, K. S. M.1 aASSIS, M. de F.1 aOLIVEIRA, E. H. C. de1 aLIMA, A. R. de1 aAQUINO, A. C. G.1 aCABRAL, R.1 aMIGLINO, M. A.  tPesquisa Veterinária Brasileira, Rio de Janeirogv. 36, n. 2, p. 119-122, fev. 2016.