02567naa a2200277 a 450000100080000000500110000800800410001910000230006024501570008326000090024052016790024965000190192865000290194765000150197665300080199165300330199965300140203265300090204670000190205570000240207470000170209870000190211570000250213470000160215977301140217520418432016-04-15       2015    bl uuuu     u00u1 u    #d1 aDELAMUTA, J. R. M.  aBradyrhizobium tropiciagri sp. nov. and Bradyrhizobium embrapense sp. nov., nitrogen-fixing symbionts of tropical forage legumes.h[electronic resource]  c2015  aAbstract: Biological nitrogen fixation is a key process for agriculture production and environmental sustainability, but there are comparatively few studies of symbionts of tropical pasture legumes, as well as few described Bradyrhizobium species, although it is the predominant 48 genus in the tropics. A detailed polyphasic study was conducted with two Bradyrhizobium strains used in commercial inoculants for tropical pastures in Brazil, CNPSo 1112T isolated from perennial soybean (Neonotonia wightii), and CNPSo 2833T from desmodium (Desmodium heterocarpon). Based on 16S-rRNA phylogeny, both strains were grouped in the B. elkanii superclade, but were not clearly clustered with any known species. MLSA of three (glnII, gyrB and recA) and five (+ atpD and dnaK) housekeeping genes confirmed that the strains are positioned in two distinct clades. Comparison with ITS sequences of described Bradyrhizobium species showed similarity lower than 93.1%, and differences were confirmed by BOX-PCR analysis. Nucleotide identity of three housekeeping genes with described species ranged from 88.1% to 96.2%. ANI of genome sequences showed values below the threshold of Bradyrhizobium species (? 90.6%) and also between the two strains (91.2%). The analysis of nifH and nodC genes positioned the two strains in a distinct clade from other Bradyrhizobium species. Morpho-physiological, genotypic and genomic data supported the description of two novel clades in the genus Bradyrhizobium, B. tropiciagri sp. nov. (type strain CNPSo 1112T =SMS 303T =BR 1009T =SEMIA 6148T =LMG 28867T) and B. embrapense sp. nov. (type strain CNPSo 2833T =CIAT 2372T =BR 2212T =SEMIA 6208T =U674T).  aBradyrhizobium  anitrogen-fixing bacteria  anodulation  aANI  aBiological nitrogen fixation  aInoculant  aMLSA1 aRIBEIRO, R. A.1 aORMEÑO-ORRILLO, E.1 aPARMA, M. M.1 aMELO, I. S. de1 aMARTÍNEZ-ROMERO, E.1 aHUNGRIA, M.  tInternational Journal of Systematic and Evolutionary Microbiology, Readinggv. 65, n. 12, p. 4424-4433, 2015.