02430naa a2200145 a 450000100080000000500110000800800410001910000220006024501170008226000090019952019460020865000100215470000190216477301010218320399562016-03-10       2015    bl uuuu     u00u1 u    #d1 aPEREIRA, L. F. P.  aCharacterization of coffee genes involved in isoprenoid and diterpene metabolic pathways.h[electronic resource]  c2015  aCoffee is consumed by more than 800 million people who enjoy its taste, flavor, and health benefits. A lthough consumption may be directly related to the quality of the coffee, little is known about the specific components of coffee, such as lipid content, that contribute to its quality. The most important lipids in coffee are the diterpenes kah-weol and cafestol, which are related to one another by their fragrance and importance to consumer health. In addition to having anticarcinogenic and intioxidant properties, they also are associated with increased concentrations of serum cholesterol. Diterpenes are one of the largest families of secondary metabolites in plants and originate from the isoprenoid pathway. Isoprenoids (also called terpenoids) are the most functionally and structurally diverse group of plant metabolites. They are synthesized in all organisms but are especially abundant and diverse in plants, with more than 40,000 compounds reported to date. Despit e the diversity of their function and structure, all isoprenoids are derived from the common five-carbon building unit iso-pentenyl diphosphate (IPP) and its isomer dimethyla llyl diphosphate (DMAPP). In higher plants, two independent pathways that are located in separate intracellular compart-ments are involved in the biosynthesis of IPP and DMAPP. In the cytosol, IPP is derived from the mevalonate (MVA) pathway. Within plastids, IPP is formed from pyruvate and glyceraldehyde 3-phosphate hrough the methylerythritol phosphate (MEP, or nonmevalonate) pathway. Although most of the genes that are associated with isoprenoid bio-synthesis have been identified using model plant species, there have been only a few studies of these genes in coffee plants. Moreover, little information about the specific formation of diterpenes is available. Here we describe the characterization of coffee genes involved in the metabolism of both isoprenoids and diterpenes.  aCafé1 aIVAMOTO, S. T.  tIn: PREEDY, V. R. Coffee in helath and disease prevention. London, UK: Elsevier, 2015. p. 45-51.