02064naa a2200373 a 450000100080000000500110000800800410001902200140006002400310007410000180010524501040012326000090022752009980023665000230123465000140125765000240127165000180129570000230131370000190133670000210135570000210137670000200139770000190141770000250143670000200146170000190148170000220150070000220152270000200154470000230156470000250158770000160161277300620162820280962017-06-20       2015    bl uuuu     u00u1 u    #d  a2156-85617 a10.4236/as.2015.630302DOI1 aMURATE, L. S.  aActivity of secondary bacterial metabolites in the control of citrus canker.h[electronic resource]  c2015  aThis study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions? activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrations were tested. Cytotoxicity effects were observed in all fractions in 50 ?g?mL?1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p &lt 0.05), reducing the incidence of citrus canker lesions.  aBiological control  aBactéria  aControle biológico  aFruta citrica1 aOLIVEIRA, A. G. de1 aHIGASHI, A. Y.1 aBARAZETTI, A. R.1 aSIMIONATO, A. S.1 aSILVA, C. S. da1 aSIMÕES, G. C.1 aSANTOS, I. M. O. dos1 aFERREIRA, M. R.1 aCELY, M. V. T.1 aNAVARRO, M. O. P.1 aFREITAS, V. F. de1 aNOGUEIRA, M. A.1 aMELLO, J. C. P. de1 aLEITE JÚNIOR, R. P.1 aANDRADE, G.  tAgricultural Sciencesgv. 6, n. 3, p. 295-303, Mar. 2015.