02324naa a2200229 a 450000100080000000500110000800800410001902400500006010000170011024501740012726000090030152015650031065000100187565000140188565000100189970000260190970000190193570000160195470000190197070000200198977300850200920274752021-04-14 2015 bl uuuu u00u1 u #d7 ahttp://dx.doi.org/10.4014/jmb.1408.080022DOI1 aGOMES, E. A. aArbuscular mycorrhizal fungal communities in the roots of maize lines contrasting for al tolerance grown in limed and non-limed Brazilian Oxisoil.h[electronic resource] c2015 aAluminum (Al) toxicity is one of the greatest limitations to agriculture in acid soils, particularly in tropical regions. Arbuscular mycorrhizal fungi (AMF) can supply plants with nutrients and give protection against Al toxicity. The aim of this work was to evaluate the effects of soil liming (i.e., reducing Al saturation) on the AMF community composition and structure in the roots of maize lines contrasting for Al tolerance. To this end, we constructed four 18S rDNA cloning libraries from L3 (Al tolerant) and L22 (Al sensitive) maize lines grown in limed and non-limed soils. A total of 790 clones were sequenced, 69% belonging to the Glomeromycota phylum. The remaining sequences were from Ascomycota, which were more prominent in the limed soil, mainly in the L3 line. The most abundant AM fungal clones were related to the family Glomeraceae represented by the genera uncultured Glomus followed by Rhizophagus and Funneliformis. However, the most abundant operational taxonomic units with 27% of the Glomeromycota clones was affiliated to genus Racocetra. This genus was present in all the four libraries, but it was predominant in the non-limed soils, suggesting that Racocetra is tolerant to Al toxicity. Similarly, Acaulospora and Rhizophagus were also present mostly in both lines in non-limed soils. The community richness of AMF in the non-limed soils was higher than the limed soil for both lines. The results suggest that the soil Al saturation was the parameter that mostly influences the AMF species composition in the soils in this study. aFungo aMicorriza aMilho1 aOLIVEIRA-PAIVA, C. A.1 aLANA, U. G. P.1 aNODA, R. W.1 aMARRIEL, I. E.1 aSOUZA, F. A. de tJournal of Microbiology and Biotechnology, Seoulgv. 25, n. 7, p. 978-987, 2015.