02359naa a2200337 a 450000100080000000500110000800800410001902200230006002400350008310000170011824501180013526000090025352013560026265000210161865000330163965000170167265000220168965000130171165000100172465000260173465000090176070000180176970000220178770000180180970000270182770000180185470000210187270000320189370000180192577300780194320168132017-08-02       2015    bl uuuu     u00u1 u    #d  a1438-7948 (online)7 a10.1007/s10142-015-0445-02DOI1 aALVES, M. S.  aDifferential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection.h[electronic resource]  c2015  aAbstract Asian soybean rust (ASR), caused by the obligate biotrophic fungus Phakopsora pachyrhizi, is one of most important diseases in the soybean (Glycine max (L.) Merr.) agribusiness. The identification and characterization of genes related to plant defense responses to fungal infection are essential to develop ASR-resistant plants. In this work, we describe four soybean genes, GmbZIP62, GmbZIP105, GmbZIPE1, and GmbZIPE2, which encode transcription factors containing a basic leucine zipper (bZIP) domain from two divergent classes, and that are responsive to P. pachyrhizi infection. Molecular phylogenetic analyses demonstrated that these genes encode proteins similar to bZIP factors responsive to pathogens. Yeast transactivation assays showed that only GmbZIP62 has strong transactivation activity in yeast. In addition, three of the bZIP transcription factors analyzed were also differentially expressed by plant defense hormones, and all were differentially expressed by fungal attack, indicating that these proteins might participate in response to ASR infection. The results suggested that these bZIP proteins are part of the plant defense response to P. pachyrhizi infection, by regulating the gene expression related to ASR infection responses. These bZIP genes are potential targets to obtain new soybean genotypes resistant to ASR.  aMicrobial growth  aPlant diseases and disorders  aSoybean rust  aDoença de planta  aFerrugem  aFungo  aPhakopsora pachyrhizi  aSoja1 aSOARES, Z. G.1 aVIDIGAL, P. M. P.1 aBARROS, E. G.1 aPODDASNOSQUI, A. M. P.1 aAOYAGI, L. N.1 aABDELNOOR, R. V.1 aMARCELINO-GUIMARÃES, F. C.1 aFIETTO, L. C.  tFunctional & Integrative Genomics, Berlingv. 15, n. 6, p. 685-696, 2015.