01410naa a2200229 a 450000100080000000500110000800800410001902200140006002400350007410000200010924501730012926000090030252006620031165000190097365000140099265000210100670000180102770000160104570000210106170000220108277300760110420143022016-03-10       2015    bl uuuu     u00u1 u    #d  a1054-54767 a10.1007/s11627-014-9660-y2DOI1 aNOGUEIRA, G. F.  aAn approach on the in vitro maintenance of sugarcane with views for conservation and monitoring of plant nuclear DNA contents via flow cytometry.h[electronic resource]  c2015  aIn vitro conservation techniques can be utilized for germplasm maintenance. However, few reports on the in vitro conservation of sugarcane species are present in the literature. The objective of this study was to subject sugarcane plants to in vitro under minimal growth conditions and to evaluate the survival, regeneration, and the monitoring of nuclear DNA content levels of the plants. Shoots from 10 sugarcane varie-ties (Saccharum spp.) were introduced into two media: MC1, consisting of half-strength Murashige and Skoog (MS) salts and 3% sorbitol, or MC2, similar to the first formulation, but additionally supplemented with 3.8 ?Mabscisicacid(ABA).  aFlow cytometry  aSugarcane  aCana de açúcar1 aPIO, L. A. S.1 aPASQUAL, M.1 aAMARAL, A. L. do1 aPEREIRA, J. E. S.  tIn Vitro Cellular & Developmental Biology. Plant.gv.51 P220-230 ,2015.