02098naa a2200277 a 450000100080000000500110000800800410001910000180006024501850007826000090026350000250027252012330029765000180153065000110154865000220155965000160158165000180159765000130161565300280162865300090165670000160166570000190168170000280170070000170172877300750174520119602017-10-11       2014    bl uuuu     u00u1 u    #d1 aHANSEL, F. A.  aThermally assisted hydrolysis and methylation (THM) analysisbA newperspective for biochemical investigation of fatty acid composition inenchytraeid tissues.h[electronic resource]  c2014  aShort communication.  aFatty acids are ubiquitous components in all organisms, and their applications in taxonomic and ecolog-ical studies of enchytraeids are scarce, and their small size (ca. 133 g fresh weight) may be the mainreason. Thermally assisted hydrolysis and methylation (THM) reaction with pyrolysis?gas chromatogra-phy (Py?GC) allow determination of fatty acid composition for small size samples. Thus, our objectivewas to test this methodology with soil enchytraeids cultured in laboratory. We used THM on-line Py?GCusing trimethylsulfonium hydroxide (TMSH) reagent to investigate fatty acid composition in tissues oftwo enchytraeid species: Enchytraeus crypticus and Enchytraeus n. sp. cultured on soil and agar. A totalof 12 fatty acids were consistently identified, ranging from C10to C16. The major fatty acids were C14:0(myristic acid) and an unsaturated C14:1. Fatty acid distribution was dependent on species and culturingmethod, suggesting the need of standardization of the culturing substrate and diet in chemosystematicsstudies. THM using TMSH provided insight on the fatty acid composition of Enchytraeus tissues and maybe promising for application in taxonomic and ecological studies of this group of neglected soil animals.  aEnchytraeidae  alipids  amass spectrometry  aOligochaeta  aphospholipids  aLipídio  aEspectrometria de massa  aFats1 aNIVA, C. C.1 aMELO, T. O. de1 aGUERRERO JÚNIOR, P. G.1 aBROWN, G. G.  tJournal of Analytical and Applied Pyrolysisgv. 110, p. 470-475, 2014.