02587naa a2200373 a 450000100080000000500110000800800410001910000170006024501510007726000090022852014650023765000150170265000180171765000180173565000210175365000280177465000260180265000090182865000240183765000220186165000240188365000120190765300200191965300170193965300180195665300220197470000270199670000190202370000130204270000190205570000220207470000190209677300980211520079312023-03-01       2015    bl uuuu     u00u1 u    #d1 aCLEMENTE, Z.  aBiomarker evaluation in fish after prolonged exposure to nano-TiO2binfluence of illumination conditions and crystal phase.h[electronic resource]  c2015  aAbstract: In this study, we evaluated the effects of prolonged exposure to two different nano-TiO2 crystal phases under different illumination conditions. Fish (Piaractus mesopotamicus) were exposed for 21 days to 100 mg/L of nano-TiO2 anatase and a mixture of anatase:rutile (80%:20%) under visible light and UV light (UVA and B, 22.47 J/cm2/h). The following oxidative stress biomarkers were monitored:concentrations of lipid hydroperoxide (LPO), carbonylated proteins (PCO), and specific activitiesof superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST). Otherbiomarkers as well as specific activities of acid phosphatase (AP), Na+, K+-ATPase and metallothioneinlevels (MT) were also evaluated. Moreover, micronucleus and comet assays were performed to assess genotoxicity. Our results showed low toxicity of nano-TiO2 to fish and lack of titanium accumulation in muscle tissue. However, it was observed the occurrence of sublethal effects that were influenced by nano-TiO2 crystal phase and illumination condition. Pure anatase caused more oxidative damage without co-exposure to UV, while the mixture anatase:rutile caused more sublethal effects when exposure occurred under UV. These findings show that the specific activity of CAT, GST, PCO levels and comet assay are useful as biomarkers of prolonged exposure to nano-TiO2. Overall, our study substantiates the development and implementation of nanoecotoxicological protocols.  aBiomarkers  aEcotoxicology  aNanoparticles  aOxidative Stress  aPiaractus mesopotamicus  aUltraviolet radiation  aPacu  aPeixe de água doce  aRaio ultravioleta  aSubstância tóxica  aToxidez  aNanopartículas  aNanotoxicity  aTitan dioxide  aUltraviolet Light1 aCASTRO, V. L. S. S. de1 aFEITOSA, L. O.1 aLIMA, R.1 aJONSSON, C. M.1 aMAIA, A. de H. N.1 aFRACETO, L. F.  tJournal of Nanoscience and Nanotechnology, Stevenson Ranchgv. 15, n. 7., p. 5424-5433, 2015.