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| Registros recuperados : 28 | |
| 4. |  | ALMEIDA, E. M. de; MONTAGNER, D. B.; ARAUJO, A. R. de; MACEDO, M. C. M.; DIFANTE, G. S.; SCARIOT, C.; PEDROSO FILHO, N. D. Produção de forragem de Brachiaria brizantha sob doses de fósforo e nitrogênio. In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 15., 2019, Campo Grande, MS. [Resumos dos trabalhos...]. Brasília, DF: Embrapa, 2019 80 p. (Embrapa Gado de Corte. Documentos, 264). Comitê Organizador: Marlene de Barros Coelho; Lenita Ramires dos Santos; Rodrigo Carvalho Alva; Lucimara Chiari; Thais Basso Amaral. p. 32-33| Biblioteca(s): Embrapa Gado de Corte. |
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| 7. | | MELO, W. L. de B.; FOSCHINI, M. M. Aplicação do equipamento - sondaleite - para o monitoramento da degradação do leite longa vida (UHT) e o teor de gorduras. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 385-389| Biblioteca(s): Embrapa Instrumentação. |
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| 17. | | NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Soja. |
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| 18. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; MACHADO, A. Q.; PIMENTA, C. B.; CASSETARI NETO, D.; JACCOUD FILHO, D. S.; BORGES, E. P.; SIQUERI, F. V.; JULIATTI, F. C.; NUNES JUNIOR, J.; CARNEIRO, L. C.; SILVA, L. H. C. P. da; MADALOSSO, M.; BALARDIN, R. S.; VENANCIO, W. S. Eficiência de fungicidas para controle de mofo-branco (Sclerotinia sclerotiorum) em soja, na safra 2013/2014 - resultados sumarizados dos ensaios cooperativos. Londrina, Embrapa Soja, 2015. 4 p. (Embrapa Soja. Circular Técnica, 109).| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Rondônia; Embrapa Roraima; Embrapa Soja; Embrapa Unidades Centrais. |
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| Registros recuperados : 28 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Soja. |
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Data corrente: |
20/02/2014 |
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Data da última atualização: |
05/04/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
NAKAYAMA, T. J.; RODRIGUES, F. A.; NEUMAIER, N.; MARCELINO-GUIMARÃES, F. C.; FARIAS, J. R. B.; OLIVEIRA, M. C. N. de; BORÉM, A.; OLIVEIRA, A. C. B. de; EMYGDIO, B. M.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; CNPSo; NORMAN NEUMAIER, CNPSO; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; JOSE RENATO BOUCAS FARIAS, CNPSO; MARIA CRISTINA NEVES DE OLIVEIRA, CNPSO; UFV; ANA CLAUDIA BARNECHE DE OLIVEIRA, CPACT; BEATRIZ MARTI EMYGDIO, CPACT; ALEXANDRE LIMA NEPOMUCENO, SRI. |
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Título: |
Reference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 1, p. 860-871, 2014. |
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DOI: |
DOI: 10.4238/2014.February.13.4 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants. |
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Palavras-Chave: |
Endogenous genes; Flooding; Housekeeping genes; Internal control genes. |
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Thesaurus NAL: |
gene expression. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97888/1/nakayama.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/97827/1/Ana-Claudia.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/112937/1/Reference-genes-soybean-..hypoxic-conditions-Incluido.pdf
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Marc: |
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024 7 $aDOI: 10.4238/2014.February.13.4$2DOI
100 1 $aNAKAYAMA, T. J.
245 $aReference genes for quantitative real-time polymerase chain reaction studies in soybean plants under hypoxic conditions.$h[electronic resource]
260 $c2014
520 $aQuantitative real-time polymerase chain reaction (RT-qPCR) is a powerful tool used to measure gene expression. However, because of its high sensitivity, the method is strongly influenced by the quality and concentration of the template cDNA and by the amplification efficiency. Relative quantification is an effective strategy for correcting random and systematic errors by using the expression level of reference gene(s) to normalize the expression level of the genes of interest. To identify soybean reference genes for use in studies of flooding stress, we compared 5 candidate reference genes (CRGs) with the NormFinder and GeNorm programs to select the best internal control. The expression stability of the CRGs was evaluated in root tissues from soybean plants subjected to hypoxic conditions. Elongation factor 1-beta and actin-11 were identified as the most appropriate genes for RT-qPCR normalization by both the NormFinder and GeNorm analyses. The expression profiles of the genes for alcohol dehydrogenase 1, sucrose synthase 4, and ascorbate peroxidase 2 were analyzed by comparing different normalizing combinations (including no normalization) of the selected reference genes. Here, we have identified potential genes for use as references for RT-qPCR normalization in experiments with soybean roots growing in O2-depleted environments, such as flooding-stressed plants.
650 $agene expression
653 $aEndogenous genes
653 $aFlooding
653 $aHousekeeping genes
653 $aInternal control genes
700 1 $aRODRIGUES, F. A.
700 1 $aNEUMAIER, N.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aFARIAS, J. R. B.
700 1 $aOLIVEIRA, M. C. N. de
700 1 $aBORÉM, A.
700 1 $aOLIVEIRA, A. C. B. de
700 1 $aEMYGDIO, B. M.
700 1 $aNEPOMUCENO, A. L.
773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 13, n. 1, p. 860-871, 2014.
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Embrapa Soja (CNPSO) |
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