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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
22/10/2012 |
Data da última atualização: |
23/02/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
GARCIA, A. R.; KAHWAGE, P. R.; GUIMARÃES, D. A. de A.; OSHASHI, O. M. |
Afiliação: |
ALEXANDRE ROSSETTO GARCIA, CPPSE; PRISCILA REIS KAHWAGE, UFPA/BELÉM; DIVA ANELIE DE ARAÚJO GUIMARÃES, UNIVERSIDADE DO PARÁ/BELÉM; OTÁVIO MITIO OHASHI, UNIVERSIDADE DO PARÁ/BELÉM. |
Título: |
Chilled semen of captive collared peccaries (Pecari tajacu): effects of preservation at 17 °C on semen quality. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Agricultural Science and Technology A, v.2, p. 921-929, 2012. |
Idioma: |
Inglês |
Conteúdo: |
The trade creation and conservation of wild species in Amazon, including collared peccaries (Pecari tajacu), may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 °C over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates (n = 65) were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact (IPM) and sperm morphology. Selected ejaculates (n = 21) were diluted (1:1) in Beltsville Thawing Solution and kept during 48 hours under controlled temperature (17 °C). Assessments were made after dilution (T0), after 24 hours (T24), and 48 hours after the onset of cooling (T48). The storage impacted on sperm survival (P < 0.05). Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before. MenosThe trade creation and conservation of wild species in Amazon, including collared peccaries (Pecari tajacu), may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 °C over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates (n = 65) were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact (IPM) and sperm morphology. Selected ejaculates (n = 21) were diluted (1:1) in Beltsville Thawing Solution and kept during 48 hours under controlled temperature (17 °C). Assessments were made after dilution (T0), after 24 hours (T24), and 48 hours after the onset of cooling (T48). The storage impacted on sperm survival (P < 0.05). Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used ... Mostrar Tudo |
Palavras-Chave: |
Amazon; Collared peccary; Semen conservation; Sperm. |
Thesaurus NAL: |
wildlife. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/68450/1/PROCI-2012.00134.pdf
|
Marc: |
LEADER 02282naa a2200217 a 4500 001 1937417 005 2023-02-23 008 2012 bl uuuu u00u1 u #d 100 1 $aGARCIA, A. R. 245 $aChilled semen of captive collared peccaries (Pecari tajacu)$beffects of preservation at 17 °C on semen quality.$h[electronic resource] 260 $c2012 520 $aThe trade creation and conservation of wild species in Amazon, including collared peccaries (Pecari tajacu), may be favored by artificial insemination, but the time of semen storage may lead to reduced fertility in sperm of some wild animals. Thus, the aim of this research was to evaluate the influence of semen storage at 17 °C over time on seminal features of peccaries in captivity. Eight adult males were sedated and underwent electroejaculation. The ejaculates (n = 65) were evaluated for volume, concentration, pH, sperm motility, vigor, and cell with intact plasma membrane intact (IPM) and sperm morphology. Selected ejaculates (n = 21) were diluted (1:1) in Beltsville Thawing Solution and kept during 48 hours under controlled temperature (17 °C). Assessments were made after dilution (T0), after 24 hours (T24), and 48 hours after the onset of cooling (T48). The storage impacted on sperm survival (P < 0.05). Semen characteristics changed throughout the storage period studied and after 48 hours storage. The decline of sperm motility was of 55.2% for 10.9%, vigor was 2.3 for 0.5 and IPM cells were of 59.0% for 15.8%. Primary defects sperm increased of 19.8% for 32.2%, secondary defects of 9.8% for 10.4% and total defects of 29.4% for 42.7%. However, within 24 hours of preservation, chilled semen peccaries presented sperm motility average rate and IMP cells levels indicative to use in assisted reproductive events. These results indicate chilled semen for 24 hours could be used in experimentally artificial insemination of peccaries, technology that still has not been performed before. 650 $awildlife 653 $aAmazon 653 $aCollared peccary 653 $aSemen conservation 653 $aSperm 700 1 $aKAHWAGE, P. R. 700 1 $aGUIMARÃES, D. A. de A. 700 1 $aOSHASHI, O. M. 773 $tJournal of Agricultural Science and Technology A$gv.2, p. 921-929, 2012.
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Embrapa Pecuária Sudeste (CPPSE) |
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