| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
|
Data corrente: |
15/06/2021 |
|
Data da última atualização: |
15/09/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. |
|
Afiliação: |
L. M. S. SIMÕES, UFLA; A. P. C. SANTOS, UFLA; M. P. BOTTINO, UFLA; E. A. LIMA, UFLA; U. R. FERNANDES, UFLA; R. E. ORLANDI, UFLA; S. A. D. RODRIGUES, UNB; F. M. CAIXETA, UNB; N. G. ALVES, UFLA; J. C. SOUZA, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; J. N. S. SALES, UFLA. |
|
Título: |
Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Theriogenology, v. 166, p. 90-96, 2021. |
|
DOI: |
https://doi.org/10.1016/j.theriogenology.2020.12.021 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. MenosThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of met... Mostrar Tudo |
|
Palavras-Chave: |
Fertilização in-vitro; IFOT; Intra-follicular. |
|
Thesagro: |
Bovino; Fertilidade Animal. |
|
Thesaurus Nal: |
Embryo (animal). |
|
Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03144naa a2200361 a 4500
001 2133325
005 2021-09-15
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.12.021$2DOI
100 1 $aSIMÕES, L. M. S.
245 $aNuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles.$h[electronic resource]
260 $c2021
520 $aThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability.
650 $aEmbryo (animal)
650 $aBovino
650 $aFertilidade Animal
653 $aFertilização in-vitro
653 $aIFOT
653 $aIntra-follicular
700 1 $aSANTOS, A. P. C.
700 1 $aBOTTINO, M. P.
700 1 $aLIMA, E. A.
700 1 $aFERNANDES, U. R.
700 1 $aORLANDI, R. E.
700 1 $aRODRIGUES, S. A. D.
700 1 $aCAIXETA, F. M.
700 1 $aALVES, N. G.
700 1 $aSOUZA, J. C.
700 1 $aQUINTAO, C. C. R.
700 1 $aCAMARGO, L. S. A.
700 1 $aDODE, M. A. N.
700 1 $aSALES, J. N. S.
773 $tTheriogenology$gv. 166, p. 90-96, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Leite (CNPGL) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Arroz e Feijão. |
|
Data corrente: |
08/10/2012 |
|
Data da última atualização: |
08/10/2012 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
COSTA, J. G. C. da; SANTIAGO, C. M.; OLIVEIRA, J. P. de; MORAES, A. da C. |
|
Afiliação: |
JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF; CARLOS MARTINS SANTIAGO, CNPAF; JAISON PEREIRA DE OLIVEIRA, CNPAF; ALESSANDRA DA CUNHA MORAES, CNPAF. |
|
Título: |
Expedições de coletas de feijão comum (Phaseolus vulgaris) no Brasil. |
|
Ano de publicação: |
2012 |
|
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. |
|
Descrição Física: |
1 CD-ROM. |
|
Idioma: |
Português |
|
Conteúdo: |
Objetivando a preservação da diversidade genética ainda existente entre os agricultores tradicionais de feijão comum, foram realizadas expedições de coleta na Região Norte do Rio Grande do Sul e nos Estados de Sergipe, Bahia e Ceará. |
|
Thesagro: |
Banco de germoplasma; Feijao; Melhoramento genético vegetal; Phaseolus vulgaris; Variedade. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/67515/1/13.pdf
|
|
Marc: |
LEADER 00959nam a2200217 a 4500
001 1935834
005 2012-10-08
008 2012 bl uuuu u00u1 u #d
100 1 $aCOSTA, J. G. C. da
245 $aExpedições de coletas de feijão comum (Phaseolus vulgaris) no Brasil.
260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2012
300 $c1 CD-ROM.
520 $aObjetivando a preservação da diversidade genética ainda existente entre os agricultores tradicionais de feijão comum, foram realizadas expedições de coleta na Região Norte do Rio Grande do Sul e nos Estados de Sergipe, Bahia e Ceará.
650 $aBanco de germoplasma
650 $aFeijao
650 $aMelhoramento genético vegetal
650 $aPhaseolus vulgaris
650 $aVariedade
700 1 $aSANTIAGO, C. M.
700 1 $aOLIVEIRA, J. P. de
700 1 $aMORAES, A. da C.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
