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| Registros recuperados : 61 | |
| 2. |  | ANDRADE, D. S.; HUNGRIA, M. Maximizing the contribution of biological nitrogen fixation in tropical legume crops. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 13., 2001, Ontario. Nitrogen fixation: global perspectives: proceedings. [S.l.]: CABI Publishing, 2002. p. 341-345. Edited by T.M. Finan, M.R. O'Brian, D.B. Layzell, J.K. Vessey, W. Newton.| Biblioteca(s): Embrapa Soja. |
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| 4. | | KASCHUK, G.; HUNGRIA, M.; ANDRADE, D. S. Diversidade de rizóbios que nodulam o feijoeiro (phaseolus vulgaris L.) sob os sistemas de plantios direto e convencional em Ponta grossa, PR. In: WORLD CONGRESS ON CONSERVATION AGRICULTURE = CONGRESSO MUNDIAL SOBRE AGRICULTURA CONSERVACIONISTA, 2., 2003, Foz do Iguaçu. Producing in harmony with nature: [proceedings ...] = Produzindo em harmonia com a natureza: [anais ...]. Ponta Grossa: Federação Brasileira de Plantio Direto na Palha, 2003. v. 2 p. 452-455. II World Congress on Conservation Agriculture, Iguassu Falls, 2003. Extended summary / posters.| Biblioteca(s): Embrapa Soja. |
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| 14. | | BALOTA, E. L.; COLOZZI-FILHO, A.; ANDRADE, D. S.; HUNGRIA, M. Biomassa microbiana e sua atividade em solos sob diferentes sistemas de preparo e sucessão de culturas. Revista Brasileira de Ciência do Solo, Viçosa, MG, v. 22, n. 4, p. 641-649, Oct./Dec. 1998.| Biblioteca(s): Embrapa Soja; Embrapa Solos; Embrapa Trigo. |
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| 15. | | BALOTA, E. L.; COLOZZI FILHO, A.; ANDRADE, D. S.; HUNGRIA, M. Avaliação microbiológica do solo sob diferentes de tipos de preparo e sucessão de culturas. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 17., 1993, Santos, SP. Resumos... (S.l.: s.n., 1993), p. 27, 1993. Resumo n.B3.012.| Biblioteca(s): Embrapa Trigo. |
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| 16. | | MIYAZAWA, M.; BALOTA, E. L.; ANDRADE, D. S.; PAVAN, M. A.; COLOZZI FILHO, A. Determinação de CO2 em análise por injeção em fluxo para estimar a biomassa microbiana do solo. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 17., 1993, Santos, SP. Resumos. (S.l.: s.n., 1993). p. 28, 1993. Resumo n.B3.013.| Biblioteca(s): Embrapa Trigo. |
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| 18. | | HUNGRIA, M.; ANDRADE, D. S.; COLOZZO-FILHO, A.; BALOTA, E. L.; CATTELAN, A. J. Ecologia microbiana em solos sob cultivo na região sul do Brasil. In: SIMPÓSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 3.; REUNIÃO DE LABORATÓRIOS PARA RECOMENDAÇÃO DE ESTIRPES DE RHIZOBIUM E BRADYRHIZOBIUM, 6., 1994, Londrina. Resumos... Londrina: IAPAR, 1994. Não paginado. Trabalho n. 22.| Biblioteca(s): Embrapa Soja. |
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| 19. | | GOMES, D. F.; CARDOSO, J. D.; MATOS, M. A.; ANDRADE, D. S.; HUNGRIA, M. Efeito da calagem e fosfatagem na diversidade de rhizobium-phaseolus em solo ácido. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas. Anais... [São Paulo]: Sociedade Brasileira de Microbiologia, 2009. Seção Resumos, ref. 2364-2. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
04/09/2012 |
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Data da última atualização: |
16/04/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GOMES, D. F.; BATISTA, J. S. da S.; SCHIAVON, A. L.; ANDRADE, D. S.; HUNGRIA, M. |
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Afiliação: |
DOUGLAS FABIANO GOMES, UEL; JESIANE STEFÂNIA DA SILVA BATISTA, UNIFIL; ALINE LUIZA SCHIAVON1, UNIFIL; DIVA SOUZA ANDRADE, IAPAR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
BMC Microbiology, London, v. 12, n. 84, p. 1-12, May 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species. MenosRhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus NAL: |
Nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
http://ainfo.cnptia.embrapa.br/digital/bitstream/item/70731/1/proteomic.bmc.microb.hungria.pdf
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Marc: |
LEADER 02200naa a2200193 a 4500
001 1932908
005 2018-04-16
008 2012 bl --- 0-- u #d
100 1 $aGOMES, D. F.
245 $aProteomic profiling of Rhizobium tropici PRF 81$bidentification of conserved and specific responses to heat stress.
260 $c2012
520 $aRhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C. Results: Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses. Conclusions: Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aBATISTA, J. S. da S.
700 1 $aSCHIAVON, A. L.
700 1 $aANDRADE, D. S.
700 1 $aHUNGRIA, M.
773 $tBMC Microbiology, London$gv. 12, n. 84, p. 1-12, May 2012.
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Embrapa Soja (CNPSO) |
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