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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
21/02/2018 |
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Data da última atualização: |
21/02/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA-FILHO, A. F. de; OSÓRIO, A. L. A. R.; JORGE, K. dos S. G.; ARAUJO, F. R.; VIDAL, C. E. S.; ARAÚJO, C. P.; ALBERTTI, L. A. G.; CAZOLA, D. de O.; FERREIRA NETO, J. S.; HEINEMANN, M. B. |
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Afiliação: |
Antonio Francisco de Souza-Filho, Faculdade de Medicina Veterinária e Zootecnia/Universidade de São Paulo - USP; Ana Luiza Alves Rosa Osório, Faculdade de Medicina Veterinária e Zootecnia - UFMS; Klaudia dos Santos Gonçalves Jorge, Centro de Ciências Biológicas e da Saúde/Universidade Federal de Mato Grosso do Sul - UFMS; FLABIO RIBEIRO ARAUJO, CNPGC; Carlos Eugênio Soto Vidal, Universidade Federal de Santa Maria - UFSM; Cristina Pires Araújo, Faculdade de Medicina Veterinária e Zootecnia - UFMS; Letícia Alves Gomes Albertti, Faculdade de Medicina Veterinária e Zootecnia - UFMS; Daniela de Oliveira Cazola, Faculdade de Medicina Veterinária e Zootecnia - UFMS; José Soares Ferreira Neto, Faculdade de Medicina Veterinária e Zootecnia/USP; Marcos Bryan Heinemann, Faculdade de Medicina Veterinária e Zootecnia/USP. |
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Título: |
Genetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Semina: Ciências Agrárias, Londrina, v. 37, n. 5, suplemento 2, p. 3719-3726, 2016 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Perfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do Brasil. |
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Conteúdo: |
Mycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group. MenosMycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity ... Mostrar Tudo |
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Palavras-Chave: |
Diversidade genética; Genetical diversity; MIRU-VNTR; Tuberculose bovina. |
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Thesagro: |
Mycobacterium Bovis. |
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Thesaurus Nal: |
Bovine tuberculosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/172941/1/28-Genetic-profiles-of-Mycobacterium-bovis-from-a-cattle-herd-in-southernmost-Brazil-2016.pdf
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Marc: |
LEADER 02626naa a2200313 a 4500
001 2087984
005 2018-02-21
008 2016 bl uuuu u00u1 u #d
100 1 $aSOUZA-FILHO, A. F. de
245 $aGenetic profiles of Mycobacterium bovis from a cattle herd in southernmost Brazil.$h[electronic resource]
260 $c2016
500 $aTítulo em português: Perfis genéticos de Mycobacterium bovis em um rebanho bovino no extremo sul do Brasil.
520 $aMycobacterium bovis is the agent of bovine tuberculosis, a disease endemic to all Brazilian states. Molecular typing techniques help to stratify and refine data, providing information that facilitates epidemiological research. In this study, MIRU-VNTR, targeting 24 loci, was employed to identify and characterize the genetic groups of M. bovis isolates obtained from an outbreak of bovine tuberculosis. Eighteen acid-fast bacilli isolates, obtained from bovine tissue samples, and reactive to the comparative cervical tuberculin test, were identified as species of the M. tuberculosis complex, and were genotyped by MIRU-VNTR with 24 primer pairs. Genotyping revealed three genetic profiles comprising one with 15 isolates (83.3%), one with two isolates (11.1%), and one profile with one unique isolate (5.6%). This distinction was achieved with the MIRU 31 primer, which resulted in clustering of two isolates into the same profile, and ETR A, B, and C, which discriminated the isolate with a unique profile. The occurrence of clustered isolates is indicative of recent transmission, whereas isolates with a unique profile suggest reactivation of latent infection. The presence of different M. bovis genotypes in the same herd suggests movement of infected animals or different sources of intra-herd infection. Use of the MIRU-VNTR molecular epidemiology technique in M. bovis isolates obtained from an outbreak of bovine tuberculosis in Rio Grande do Sul state demonstrated the genetic diversity of circulating strains, despite the presence of a predominant group.
650 $aBovine tuberculosis
650 $aMycobacterium Bovis
653 $aDiversidade genética
653 $aGenetical diversity
653 $aMIRU-VNTR
653 $aTuberculose bovina
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. dos S. G.
700 1 $aARAUJO, F. R.
700 1 $aVIDAL, C. E. S.
700 1 $aARAÚJO, C. P.
700 1 $aALBERTTI, L. A. G.
700 1 $aCAZOLA, D. de O.
700 1 $aFERREIRA NETO, J. S.
700 1 $aHEINEMANN, M. B.
773 $tSemina: Ciências Agrárias, Londrina$gv. 37, n. 5, suplemento 2, p. 3719-3726, 2016
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Solos. |
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Data corrente: |
26/01/2011 |
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Data da última atualização: |
02/05/2014 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
FERREIRA, G. B.; SILVA, M. S. L. da; COSTA, M. B. B. da; MOURA, M. M.; GAVA, C. A. T.; MENDONÇA, C. E. S.; CHAVES, V. C. |
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Afiliação: |
Gizelia Barbosa Ferreira, Universidade Federal de São Carlos; MARIA SONIA LOPES DA SILVA, CNPS; Manoel Baltasar Baptista da Costa, Universidade Federal de São Carlos; Márcia Moura Moreira, Bolsista do CNPq; CARLOS ALBERTO TUAO GAVA, CPATSA; Cláudio Evangelista Santos Mendonça, Universidade Federal Rural de Pernambuco; Vanessa Carine Chaves. |
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Título: |
Carbono orgânico total em solos sob influência de barragens subterrâneas no semiárido da Paraíba, Brasil. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29., 2010, Guarapari, ES. Anais... Guarapari, ES: FertBio, 2010. |
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Idioma: |
Português |
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Palavras-Chave: |
Captação e armazenamento de água de chuva; Qualidade do solo; Sistemas de base ecológica. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25961/1/1604.pdf
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Marc: |
LEADER 00791nam a2200205 a 4500
001 1874509
005 2014-05-02
008 2010 bl uuuu u00u1 u #d
100 1 $aFERREIRA, G. B.
245 $aCarbono orgânico total em solos sob influência de barragens subterrâneas no semiárido da Paraíba, Brasil.$h[electronic resource]
260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29., 2010, Guarapari, ES. Anais... Guarapari, ES: FertBio$c2010
653 $aCaptação e armazenamento de água de chuva
653 $aQualidade do solo
653 $aSistemas de base ecológica
700 1 $aSILVA, M. S. L. da
700 1 $aCOSTA, M. B. B. da
700 1 $aMOURA, M. M.
700 1 $aGAVA, C. A. T.
700 1 $aMENDONÇA, C. E. S.
700 1 $aCHAVES, V. C.
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