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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
26/07/2011 |
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Data da última atualização: |
05/12/2012 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SARDINA, M. T.; ROSA, A. J. de M.; DAVOLI, R.; BRAGLIA, S.; PORTOLANO, B. |
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Afiliação: |
M. T. SARDINA, UNIVERSITY OF PALERMO; ARTUR JORDAO DE MAGALHAES ROSA, CPAC; R. DAVOLI, UNIVERSITY OF BOLOGNA; S. BRAGLIA, UNIVERSITY OF BOLOGNA; B. PORTOLANO, UNIVERSITY OF PALERMO. |
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Título: |
Polymorphisms of beta-lactoglobulin promoter region in three sicilian goat breeds. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 39, n. 3, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Several beta-lactoglobulin (BLG) polymorphisms have been described within the proximal promoter region and coding region of the caprine gene, although no genetic variants affecting the protein amino acid composition and/or expression level have been characterized so far. Binding sites for several transcription factors (TFs) are present in the BLG promoter region. The aims of this work were to sequence the full-length promoter region of three Sicilian goat breeds in order to identify polymorphisms, analyze the identified haplotypes, search for differences between breeds for the presence of polymorphisms in this gene region, search for putative TFs binding sites, and check if polymorphisms lay within the identified TFs binding sites. The promoter region of BLG gene in Sicilian goat breeds showed high level of polymorphism due to the presence of 36 single nucleotide polymorphisms (SNPs). Association between polymorphic sites was computed within the whole sample analyzed and 18 haplotypes were inferred. Binding sites for three milk protein binding factors (MPBFs) and four nuclear factor-I (NF-I) were found within BLG promoter region based on the ovine sequence. The identification of some SNPs within TFs binding sites allowed hypothesizing the loss of TFs. Further studies are in progress to evaluate the effect of these mutations on binding affinity of TFs, the functional interaction of the TFs with the goat BLG promoter, and the relationship of the polymorphisms with BLG gene expression and milk production and composition. MenosSeveral beta-lactoglobulin (BLG) polymorphisms have been described within the proximal promoter region and coding region of the caprine gene, although no genetic variants affecting the protein amino acid composition and/or expression level have been characterized so far. Binding sites for several transcription factors (TFs) are present in the BLG promoter region. The aims of this work were to sequence the full-length promoter region of three Sicilian goat breeds in order to identify polymorphisms, analyze the identified haplotypes, search for differences between breeds for the presence of polymorphisms in this gene region, search for putative TFs binding sites, and check if polymorphisms lay within the identified TFs binding sites. The promoter region of BLG gene in Sicilian goat breeds showed high level of polymorphism due to the presence of 36 single nucleotide polymorphisms (SNPs). Association between polymorphic sites was computed within the whole sample analyzed and 18 haplotypes were inferred. Binding sites for three milk protein binding factors (MPBFs) and four nuclear factor-I (NF-I) were found within BLG promoter region based on the ovine sequence. The identification of some SNPs within TFs binding sites allowed hypothesizing the loss of TFs. Further studies are in progress to evaluate the effect of these mutations on binding affinity of TFs, the functional interaction of the TFs with the goat BLG promoter, and the relationship of the polymorphisms with BLG gene exp... Mostrar Tudo |
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Palavras-Chave: |
Beta lactoglobulina. |
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Thesagro: |
Genética; Polimorfismo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02105naa a2200205 a 4500
001 1896808
005 2012-12-05
008 2012 bl uuuu u00u1 u #d
100 1 $aSARDINA, M. T.
245 $aPolymorphisms of beta-lactoglobulin promoter region in three sicilian goat breeds.
260 $c2012
520 $aSeveral beta-lactoglobulin (BLG) polymorphisms have been described within the proximal promoter region and coding region of the caprine gene, although no genetic variants affecting the protein amino acid composition and/or expression level have been characterized so far. Binding sites for several transcription factors (TFs) are present in the BLG promoter region. The aims of this work were to sequence the full-length promoter region of three Sicilian goat breeds in order to identify polymorphisms, analyze the identified haplotypes, search for differences between breeds for the presence of polymorphisms in this gene region, search for putative TFs binding sites, and check if polymorphisms lay within the identified TFs binding sites. The promoter region of BLG gene in Sicilian goat breeds showed high level of polymorphism due to the presence of 36 single nucleotide polymorphisms (SNPs). Association between polymorphic sites was computed within the whole sample analyzed and 18 haplotypes were inferred. Binding sites for three milk protein binding factors (MPBFs) and four nuclear factor-I (NF-I) were found within BLG promoter region based on the ovine sequence. The identification of some SNPs within TFs binding sites allowed hypothesizing the loss of TFs. Further studies are in progress to evaluate the effect of these mutations on binding affinity of TFs, the functional interaction of the TFs with the goat BLG promoter, and the relationship of the polymorphisms with BLG gene expression and milk production and composition.
650 $aGenética
650 $aPolimorfismo
653 $aBeta lactoglobulina
700 1 $aROSA, A. J. de M.
700 1 $aDAVOLI, R.
700 1 $aBRAGLIA, S.
700 1 $aPORTOLANO, B.
773 $tMolecular Biology Reports$gv. 39, n. 3, 2012.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
16/10/2008 |
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Data da última atualização: |
03/02/2009 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ALVES, A.; SETTER, T.; FREGENE, M.; FERGUSON, M.; DUQUE, L.; MUTEGI, R.; MKAMILO, G. |
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Afiliação: |
Alfredo Augusto Cunha Alves, CNPMF; Tim Setter, Cornell University; Martin Fregene, CIAT; Morag Ferguson, IITA; Luis Duque, Cornell University; Rosemary Mutegi, ICRISAT-Nairobi; Geoffrey Mkamilo, ARI-Tanzania. |
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Título: |
Traits related to drought tolerance mechanisms in cassava. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 53. |
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Idioma: |
Inglês |
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Conteúdo: |
Contrasting cassava varieties were screened in semi-arid environments to characterise genotypes for drought tolerance traits. The following hypotheses were supported: 1) genotypes that accumulated substantial stem starch reserves may be more able to sustain meristems and other respiring organs during a prolonged stress; 2) genotypes that maintain deep fibrous root growth perform better in drought; 3) genotypes that maintain partiotioning to storage roots and have high harvest index yield better in drought; and 4) genotypes that are more effective in closing stomata are more able to maintain water status and retain leaves. Leaf conductance, leaf retention, leaf size, harvest index, ABA, sugars and starch accumulation in the leaves and stems were differentially affected in the cassava varieties and highly correlated to yield performance under water deficit. The different responses to water deficit for some parameters has helped us to better discriminate varieties as tolerant or susceptible to drought and to define the best traits for selecting in breeding programmes. Three segregating populations with a total of 370 individuals were produced. A genotypic parental screen has revealed 168 polymorphic SSRs in COL1734 x VEN77 and 147 in COL1468 x BRA255, from 307 SSRs screened. Genotyping and phenotyping of the mapping populations are underway. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01970naa a2200193 a 4500
001 1637241
005 2009-02-03
008 2008 bl uuuu u00u1 u #d
100 1 $aALVES, A.
245 $aTraits related to drought tolerance mechanisms in cassava.
260 $c2008
520 $aContrasting cassava varieties were screened in semi-arid environments to characterise genotypes for drought tolerance traits. The following hypotheses were supported: 1) genotypes that accumulated substantial stem starch reserves may be more able to sustain meristems and other respiring organs during a prolonged stress; 2) genotypes that maintain deep fibrous root growth perform better in drought; 3) genotypes that maintain partiotioning to storage roots and have high harvest index yield better in drought; and 4) genotypes that are more effective in closing stomata are more able to maintain water status and retain leaves. Leaf conductance, leaf retention, leaf size, harvest index, ABA, sugars and starch accumulation in the leaves and stems were differentially affected in the cassava varieties and highly correlated to yield performance under water deficit. The different responses to water deficit for some parameters has helped us to better discriminate varieties as tolerant or susceptible to drought and to define the best traits for selecting in breeding programmes. Three segregating populations with a total of 370 individuals were produced. A genotypic parental screen has revealed 168 polymorphic SSRs in COL1734 x VEN77 and 147 in COL1468 x BRA255, from 307 SSRs screened. Genotyping and phenotyping of the mapping populations are underway.
700 1 $aSETTER, T.
700 1 $aFREGENE, M.
700 1 $aFERGUSON, M.
700 1 $aDUQUE, L.
700 1 $aMUTEGI, R.
700 1 $aMKAMILO, G.
773 $tIn: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 53.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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