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Biblioteca(s):  Embrapa Cerrados.
Data corrente:  26/07/2011
Data da última atualização:  05/12/2012
Tipo da produção científica:  Artigo em Periódico Indexado
Autoria:  SARDINA, M. T.; ROSA, A. J. de M.; DAVOLI, R.; BRAGLIA, S.; PORTOLANO, B.
Afiliação:  M. T. SARDINA, UNIVERSITY OF PALERMO; ARTUR JORDAO DE MAGALHAES ROSA, CPAC; R. DAVOLI, UNIVERSITY OF BOLOGNA; S. BRAGLIA, UNIVERSITY OF BOLOGNA; B. PORTOLANO, UNIVERSITY OF PALERMO.
Título:  Polymorphisms of beta-lactoglobulin promoter region in three sicilian goat breeds.
Ano de publicação:  2012
Fonte/Imprenta:  Molecular Biology Reports, v. 39, n. 3, 2012.
Idioma:  Inglês
Conteúdo:  Several beta-lactoglobulin (BLG) polymorphisms have been described within the proximal promoter region and coding region of the caprine gene, although no genetic variants affecting the protein amino acid composition and/or expression level have been characterized so far. Binding sites for several transcription factors (TFs) are present in the BLG promoter region. The aims of this work were to sequence the full-length promoter region of three Sicilian goat breeds in order to identify polymorphisms, analyze the identified haplotypes, search for differences between breeds for the presence of polymorphisms in this gene region, search for putative TFs binding sites, and check if polymorphisms lay within the identified TFs binding sites. The promoter region of BLG gene in Sicilian goat breeds showed high level of polymorphism due to the presence of 36 single nucleotide polymorphisms (SNPs). Association between polymorphic sites was computed within the whole sample analyzed and 18 haplotypes were inferred. Binding sites for three milk protein binding factors (MPBFs) and four nuclear factor-I (NF-I) were found within BLG promoter region based on the ovine sequence. The identification of some SNPs within TFs binding sites allowed hypothesizing the loss of TFs. Further studies are in progress to evaluate the effect of these mutations on binding affinity of TFs, the functional interaction of the TFs with the goat BLG promoter, and the relationship of the polymorphisms with BLG gene exp... Mostrar Tudo
Palavras-Chave:  Beta lactoglobulina.
Thesagro:  Genética; Polimorfismo.
Categoria do assunto:  --
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Cerrados (CPAC)
Biblioteca ID Origem Tipo/Formato Classificação Cutter Registro Volume Status URL
CPAC32707 - 1UPCAP - PPS1447S1447
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Biblioteca(s):  Embrapa Mandioca e Fruticultura.
Data corrente:  16/10/2008
Data da última atualização:  03/02/2009
Tipo da produção científica:  Resumo em Anais de Congresso
Autoria:  ALVES, A.; SETTER, T.; FREGENE, M.; FERGUSON, M.; DUQUE, L.; MUTEGI, R.; MKAMILO, G.
Afiliação:  Alfredo Augusto Cunha Alves, CNPMF; Tim Setter, Cornell University; Martin Fregene, CIAT; Morag Ferguson, IITA; Luis Duque, Cornell University; Rosemary Mutegi, ICRISAT-Nairobi; Geoffrey Mkamilo, ARI-Tanzania.
Título:  Traits related to drought tolerance mechanisms in cassava.
Ano de publicação:  2008
Fonte/Imprenta:  In: CGIAR GENERATION CHALLENGE PROGRAMME ANNUAL RESEARCH MEETING, 2008, Bangkok, Thailand. Poster abstracts... Mexico, DF: Generation Challenge Programme, 2008. p. 53.
Idioma:  Inglês
Conteúdo:  Contrasting cassava varieties were screened in semi-arid environments to characterise genotypes for drought tolerance traits. The following hypotheses were supported: 1) genotypes that accumulated substantial stem starch reserves may be more able to sustain meristems and other respiring organs during a prolonged stress; 2) genotypes that maintain deep fibrous root growth perform better in drought; 3) genotypes that maintain partiotioning to storage roots and have high harvest index yield better in drought; and 4) genotypes that are more effective in closing stomata are more able to maintain water status and retain leaves. Leaf conductance, leaf retention, leaf size, harvest index, ABA, sugars and starch accumulation in the leaves and stems were differentially affected in the cassava varieties and highly correlated to yield performance under water deficit. The different responses to water deficit for some parameters has helped us to better discriminate varieties as tolerant or susceptible to drought and to define the best traits for selecting in breeding programmes. Three segregating populations with a total of 370 individuals were produced. A genotypic parental screen has revealed 168 polymorphic SSRs in COL1734 x VEN77 and 147 in COL1468 x BRA255, from 307 SSRs screened. Genotyping and phenotyping of the mapping populations are underway.
Categoria do assunto:  --
Marc:  Mostrar Marc Completo
Registro original:  Embrapa Mandioca e Fruticultura (CNPMF)
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