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| Registros recuperados : 9 | |
| 1. |  | ANDREAZZA, D.; BOOS, G. S.; BOABAID, F. M.; WOUTERS, A. T. B.; SOUZA, S. O. Caracterização histológica e imuno-histoquímica das lesões de tuberculose em bovinos e de linfadenite granulomatosa em suínos. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n.2, p.129-136, fev. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | BANDARRA, P. M.; OLIVEIRA, L. G. de; DALTO, A. C.; BOABAID, F. M.; JUFFO, G.; RIET-CORREA, F.; DRIEMEIER, D.; CRUZ, C. E. F. da. Sheep production as a Senecio spp. control tool. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 32, n. 10, p. 1017-1022, out. 2012.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 3. | | BOABAID, F. M.; FERREIRA, E. V.; ARRUDA, L. P.; GASPARETTO, N. D.; SOUZA, R. L. de; SILVA, M. C. da; DUTRA, V.; NAKAZATO, L.; COLODEL, E. M. Conidiobolomicose em ovinos no Estado de Mato Grosso. Pesquisa Veterinária Brasileira, v. 28, n. 1, p. 77-81, jan. 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 4. | | BOABAID, F. M.; OLIVEIRA, L. G. S.; DALTO, A. G. C.; BANDARRA, P. M.; SOUZA, F. S.; SONNE, L.; DRIEMEIER, D. Achados clínico-patológicos e métodos de controle da intoxicação por Pteridium (aquilinum) arachnoideum em uma propriedade do Rio Grande do Sul. Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n 8, p. 1584-1596, agosto 2018 Título em inglês: Clinical and pathological findings and control methods of the poisoning by Pteridium (aquilinum) arachnoideum in a farm of Rio Grande do Sul, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | MARTINS, K. P. F.; FONSECA, T. R. S.; SILVA, E. S.; MUNHOZ, T. C. P.; DIAS, G. H. S.; GALIZA, G. J. N.; OLIVEIRA, L. G. S.; BOABAID, F. M. Bócio em bovinos. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1030-1037, junho 2018 Título em inglês: Goiter in cattle.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 6. | | MENDONÇA, F. S.; EVÊNCIO NETO, J.; ESTEVÃO, L. R. M.; MELO, L. E. H.; FREITAS, S. H.; ARRUDA, L. P.; BOABAID, F. M.; COLODEL, E. M. Aspectos clínicos da intoxicação experimental pelas favas de Stryphnodendron fissuratum (Leg. Mimosoideae) em caprinos. Pesquisa Veterinária Brasíleira, Rio de Janeiro, v. 30, n. 3, p. 203-210, mar., 2010.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 7. | | NAKAGAKI, K. Y. R.; LIMA, P. A.; UTIUMI, K. U.; PIRES, M. A. M.; ZANATTA, R.; BOABAID, F. M.; COLODEL, E. M.; RAYMUNDO, D. L. Transitional cell carcinoma of urinary bladder with metastasis in lumbar vertebrae and spinal cord compression in an ocelot. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n.1, p.75-79, jan. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 8. | | REIS, M. de O.; CAPRIOLI, R. A.; LAISSE, C. J. M.; GUIMARÃES, L. L. B.; ANDRADE, C. P. de; BOABAID, F. M.; SONNE, L.; DRIEMEIER, D. Surto de leptospirose em bezerros criados em resteva de arroz. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 9, p.937-940, setembro. 2017. Título em inglês: Leptospirosis outbreak in calves maintained in a rice stubble field.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | REIS, M. O.; CRUZ, R. A. S.; BASSUINO, D. M.; BOABAID, F. M.; OLIVEIRA, L. G. S.; MELLO, L. S.; SONNE, L.; DRIEMEIER, D. Hypomyelinogenesis associated with transplacental poisoning by Sida carpinifolia (Malvaceae) in fetus and newborn calves. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 7, p. 1371-1375, julho 2018 Título em inglês: Hipomielinogênese associada à intoxicação transplacentária por Sida carpinifolia (Malvaceae) em fetos e neonatos bovinos.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 9 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
29/05/2023 |
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Data da última atualização: |
05/04/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MOREIRA, V. J. V.; PINHEIRO, D. H.; LOURENCO, I. T.; BASSO, M. F.; LISEI-DE-SA, M. E.; SILVA, M. C. M. da; DANCHIN, E. G. J.; GUIMARAES, P. M.; GRYNBERG, P.; BRASILEIRO, A. C. M.; MACEDO, L. L. P. de; MORGANTE, C. V.; ENGLER, J. de A.; SA, M. F. G. de. |
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Afiliação: |
VALDEIR JUNIO VAZ MOREIRA, Biotechnology Center, PPGBCM, UFRGS; DANIELE HELOÍSA PINHEIRO, National Institute of Science and Technology; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; MARCOS FERNANDO BASSO, National Institute of Science and Technology; MARIA E. LISEI-DE-SA, National Institute of Science and Technology; MARIA CRISTINA MATTAR DA SILVA, Cenargen; ETIENNE G. J. DANCHIN, National Institute of Science and Technology; PATRICIA MESSEMBERG GUIMARAES, Cenargen; PRISCILA GRYNBERG, Cenargen; ANA CRISTINA MIRANDA BRASILEIRO, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; JANICE DE ALMEIDA ENGLER, National Institute of Science and Technology; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
In planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Journal of Pest Science, v. 97, p. 411-427, 2024. |
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DOI: |
https://doi.org/10.1007/s10340-023-01623-7 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Maria C. M. Silva; Leonardo L. P. Macedo; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
Meloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing. MenosMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disor... Mostrar Tudo |
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Palavras-Chave: |
DsRNA; Host-induced gene silencing; Interações planta-nematóide; Nematoide das galhas; Plant-nematode interactions; Root-knot nematode. |
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Thesagro: |
Meloidogyne Incognita; Nematóide; Parasito de Planta; Planta. |
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Thesaurus NAL: |
Nematode biology; Nematode control; Plant parasitic nematodes. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/264479/1/In-planta-RNAi-targeting-Meloidogyne-incognita-Minc16803-gene-perturbs-nematode-parasitism-and-reduces-plant-susceptibility.pdf
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Marc: |
LEADER 03407naa a2200457 a 4500
001 2154064
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008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10340-023-01623-7$2DOI
100 1 $aMOREIRA, V. J. V.
245 $aIn planta RNAi targeting Meloidogyne incognita Minc16803 gene perturbs nematode parasitism and reduces plant susceptibility.$h[electronic resource]
260 $c2024
500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Maria C. M. Silva; Leonardo L. P. Macedo; Maria Fatima Grossi-de-Sa.
520 $aMeloidogyne incognita is one of the most important plant-parasitic nematodes (PPNs) causing severe crop losses worldwide. Plants have evolved complex defense mechanisms to respond to PPNs attacks. Conversely, PPNs have evolved infection mechanisms that involve the secretion of effector proteins into host plants to suppress immune responses and facilitate para- sitism. Therefore, effector genes are attractive targets for the genetic improvement of plant resistance to M. incognita. In this study, we functionally characterized the Minc16803 (Minc3s00746g16803) putative effector gene to evaluate its role during plant-nematode interactions. First, we found that the Minc16803 gene is expressed in all nematode life stages and encodes a protein with an N-terminal signal peptide for secretion, a motif characteristic of effector proteins and with the absence of transmembrane domain. In addition, our data demonstrated that transgenic Arabidopsis thaliana lines overexpressing a Minc16803-dsRNA efficiently downregulated the Minc16803 transcripts in infecting nematodes. Furthermore, transgenic lines were significantly less susceptible to M. incognita compared to wild-type control plants. The number of galls per plant was reduced by up to 84%, while the number of egg masses per plant decreased by up to 93.3%. Moreover, galls and feed- ing sites in the roots of transgenic lines were smaller than those in the control plants. Histological analysis revealed giant cells without cytoplasm, disordered neighboring cells, and malformed maturing nematodes in transgenic galls. Curiously, numerous hatching ppJ2 juveniles were often observed near the female body within the transgenic root tissues before egg mass extrusion. All findings strongly suggest that Minc16803 gene is a promising target to engineer agricultural crops for M. incognita resistance through host-induced gene silencing.
650 $aNematode biology
650 $aNematode control
650 $aPlant parasitic nematodes
650 $aMeloidogyne Incognita
650 $aNematóide
650 $aParasito de Planta
650 $aPlanta
653 $aDsRNA
653 $aHost-induced gene silencing
653 $aInterações planta-nematóide
653 $aNematoide das galhas
653 $aPlant-nematode interactions
653 $aRoot-knot nematode
700 1 $aPINHEIRO, D. H.
700 1 $aLOURENCO, I. T.
700 1 $aBASSO, M. F.
700 1 $aLISEI-DE-SA, M. E.
700 1 $aSILVA, M. C. M. da
700 1 $aDANCHIN, E. G. J.
700 1 $aGUIMARAES, P. M.
700 1 $aGRYNBERG, P.
700 1 $aBRASILEIRO, A. C. M.
700 1 $aMACEDO, L. L. P. de
700 1 $aMORGANTE, C. V.
700 1 $aENGLER, J. de A.
700 1 $aSA, M. F. G. de
773 $tJournal of Pest Science$gv. 97, p. 411-427, 2024.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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