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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
08/11/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MONTEIRO, J. P.; SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; ANDRÉ, W. P. P.; RIBEIRO, W. L. C.; MELO, A. C. F. L.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. |
Afiliação: |
JOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) -Sobral, CE, Brazil; Gracielle Araújo Frota; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Ana Carolina Fonseca Lindoso Melo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil. |
Título: |
Molecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588. |
Idioma: |
Inglês |
Notas: |
In conjunction with 53rd MSPTM Annual Conference. |
Conteúdo: |
Abstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period. MenosAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a sh... Mostrar Tudo |
Palavras-Chave: |
Beta tubulin; Dosage effects; Resistance to anthelmintics; SNP. |
Thesagro: |
Anti-Helmíntico; Haemonchus contortus; Resistência química. |
Thesaurus Nal: |
Anthelmintics; Drug resistance; Oxfendazole. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166452/1/CNPC-2017-Molecular.pdf
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Marc: |
LEADER 02735nam a2200349 a 4500 001 2079254 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aMONTEIRO, J. P. 245 $aMolecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588.$c4588 500 $aIn conjunction with 53rd MSPTM Annual Conference. 520 $aAbstract: Haemonchus contortus is one of the main small ruminant parasites in tropical areas and its control is traditionally done through the utilization of synthetic anthelmintics such as benzimidazoles (BZ). BZ resistance is associated to single nucleotide polymorphisms (SNP) located in the Beta-tubulin isotype 1 gene: F200Y, F167Y and E198A. Here we describe the rise of resistant mutations within a completely susceptible isolate (Inbred-susceptible-Edinburgh - ISE) in an experimental infection model in 3 sheep treated with increasing oxfendazole doses. After initial infection and rise in fecal egg counts, the drug regimen started with subdoses as low as 30% of the recommended dosage and were gradually increased to 100% over the course of a year. Experimental animals were infected every 1.5 months with 5,000 L3 larvae cultured from feces collected one week after each treatment. After an initial period when resistance was only detected in the F200Y locus, both resistant polymorphisms (F200Y and F167Y) were detected in increasing frequencies after each treatment. Eventually resistance at F200Y stationed at frequencies around 70% and decreased while resistance frequencies at F167Y kept rising and finally stationed at around 75%. Resistance at E198A was never detected in the studied period. Our study provide insights into the speed as to which each polymorphism may rise as BZ dosage increases converting a susceptible parasite population into a highly resistant one within a short time period. 650 $aAnthelmintics 650 $aDrug resistance 650 $aOxfendazole 650 $aAnti-Helmíntico 650 $aHaemonchus contortus 650 $aResistência química 653 $aBeta tubulin 653 $aDosage effects 653 $aResistance to anthelmintics 653 $aSNP 700 1 $aSANTOS, J. M. L. dos 700 1 $aVASCONCELOS, J. F. 700 1 $aFROTA, G. A. 700 1 $aANDRÉ, W. P. P. 700 1 $aRIBEIRO, W. L. C. 700 1 $aMELO, A. C. F. L. 700 1 $aTEIXEIRA, M. 700 1 $aVIEIRA, L. da S. 700 1 $aBEVILAQUA, C. M. L.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
11/01/2023 |
Data da última atualização: |
13/01/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ALMEIDA, J. R. M. de; SOUZA, M. de A.; CARNEIRO, C. V. G. C.; ABDELNUR, P. V. |
Afiliação: |
JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE; MIRIA DE ALMEIDA SOUZA, CNPAE/ FEDERAL UNIVERSITY OF GOIÁS; CLARA VIDA GALVÃO CORRÊA CARNEIRO, CNPAE/ FEDERAL UNIVERSITY OF GOIÁS; PATRÍCIA VERARDI ABDELNUR, CNPAE/ FEDERAÇ UNIVERSITY OF GOIÁS. |
Título: |
Analysis of Komagataella phaffii metabolites using mass spectrometry. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
In: IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3., 2022, Rio de Janeiro. Book of abstracts. [S/l]: Sociedade Brasileira de Espectrometria de Massas, 2022. |
Páginas: |
p. 367 |
Idioma: |
Inglês |
Conteúdo: |
The use of fermentation of lignocellulosic biomass by Komagataella phaffii produces biomolecules through the conversion of xylose, as ethylene glycol building block for the production of molecules in the chemical and pharmaceutical industry[1, 2]. The synthetic way of producing ethylene glycol from xylose, being identified and characterized by metabolomics-based mass spectrometry[2,3]. |
Palavras-Chave: |
Building blocks. |
Thesaurus NAL: |
Fermentation; Mass spectrometry; Metabolomics; Xylose. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150895/1/Analysis-of-Komagataella.pdf
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Marc: |
LEADER 01119nam a2200217 a 4500 001 2150895 005 2023-01-13 008 2022 bl uuuu u00u1 u #d 100 1 $aALMEIDA, J. R. M. de 245 $aAnalysis of Komagataella phaffii metabolites using mass spectrometry.$h[electronic resource] 260 $aIn: IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3., 2022, Rio de Janeiro. Book of abstracts. [S/l]: Sociedade Brasileira de Espectrometria de Massas$c2022 300 $ap. 367 520 $aThe use of fermentation of lignocellulosic biomass by Komagataella phaffii produces biomolecules through the conversion of xylose, as ethylene glycol building block for the production of molecules in the chemical and pharmaceutical industry[1, 2]. The synthetic way of producing ethylene glycol from xylose, being identified and characterized by metabolomics-based mass spectrometry[2,3]. 650 $aFermentation 650 $aMass spectrometry 650 $aMetabolomics 650 $aXylose 653 $aBuilding blocks 700 1 $aSOUZA, M. de A. 700 1 $aCARNEIRO, C. V. G. C. 700 1 $aABDELNUR, P. V.
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