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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
04/01/2024 |
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Data da última atualização: |
04/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, F. C.; CLEMENTE, A. C. S.; CAIXETA, F.; ROSA, S. D. V. F. da. |
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Afiliação: |
F. C. SANTOS, UDI PESQUISA & DESENVOLVIMENTO; A. C. S. CLEMENTE, UNIVERSIDADE FEDERAL DE LAVRAS; F. CAIXETA, GENERAL MILLS BRASIL ALIMENTOS LTDA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa. |
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Título: |
Endo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n. 4 p. 11719-11728, 2015. |
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DOI: |
http://dx.doi.org/10.4238/2015.October.2.5 |
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Idioma: |
Inglês |
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Conteúdo: |
Coffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed maturation. MenosCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed matur... Mostrar Tudo |
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Thesaurus Nal: |
Coffea arabica var. arabica; Developmental stages; Organogenesis; Seed maturation. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/260321/1/Endo-mannanase.pdf
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Marc: |
LEADER 02224naa a2200217 a 4500
001 2160448
005 2024-01-04
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2015.October.2.5$2DOI
100 1 $aSANTOS, F. C.
245 $aEndo-β-mannanase and β-tubulin gene expression during the final phases of coffee seed maturation.$h[electronic resource]
260 $c2015
520 $aCoffee seeds begin to develop shortly after fertilization and can take 6 to 8 months to complete their formation, a period during which all the characteristics of the mature seed are determined, directly influencing physiological quality. However, little is known about the molecular mechanisms that act during coffee seed maturation. The objective of the current study was to analyze expression of the beta-tubulin (TUB) and endo-beta-mannanase (MAN) genes during different phases at the end of development and in different tissues of Coffea arabica seeds. The transcription levels of the TUB and MAN genes were quantified in a relative manner using qRT-PCR in whole seeds, and dissected into embryos and endosperms at different developmental stages. Greater expression of MAN was observed in whole seeds and in endosperms during the green stage, and in the embryo during the over-ripe stage. High TUB gene expression was observed in whole seeds during the green stage and, in the embryos, there were peaks in expression during the over-ripe stage. In endosperms, the peak of expression occurred in both the green stage and in the cherry stage. These results suggest participation of endo-beta-mannanase during the initial seed developmental stages, and in the stages of physiological maturity in the embryo tissues. TUB gene expression varied depending on the developmental stage and section of seed analyzed, indicating the participation of beta-tubulin during organogenesis and coffee seed maturation.
650 $aCoffea arabica var. arabica
650 $aDevelopmental stages
650 $aOrganogenesis
650 $aSeed maturation
700 1 $aCLEMENTE, A. C. S.
700 1 $aCAIXETA, F.
700 1 $aROSA, S. D. V. F. da
773 $tGenetics and Molecular Research$gv. 14, n. 4 p. 11719-11728, 2015.
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Registro original: |
Embrapa Café (CNPCa) |
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Biblioteca(s): |
Embrapa Agrobiologia; Embrapa Agroindústria de Alimentos. |
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Data corrente: |
21/06/2022 |
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Data da última atualização: |
21/06/2022 |
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Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
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Autoria: |
AQUINO, A. M. de; TEIXEIRA, A. J.; FONSECA, M. J. de O.; ASSIS, R. L. de; OZASSA, T. I. (ed.). |
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Afiliação: |
ADRIANA MARIA DE AQUINO, CNPAB; ALEXANDRE JACINTHO TEIXEIRA; MARCOS JOSE DE OLIVEIRA FONSECA, CTAA; RENATO LINHARES DE ASSIS, CNPAB; TERESA YOSHIKO OZASSA, Viveiro Ninkasi, Teresópolis, RJ. |
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Título: |
Produção de lúpulo na Região Serrana Fluminense: manual de boas práticas. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Nova Friburgo: Associação Comercial, Industrial e Agrícola de Nova Friburgo - ACIANF, 2022. |
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Páginas: |
140 p. |
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ISBN: |
978-65-992575-1-3 |
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Idioma: |
Português |
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Conteúdo: |
Rede para o fomento à cultura do lúpulo na região serrana fluminense. Zoneamento Agroecológico preliminar para a cultura do lúpulo no Estado do Rio de Janeiro. Mudas de Lúpulo (Humulus lupulus L.). Manejo e condução da cultura do lúpulo. Recomendações preliminares de calagem e adubação para a cultura do lúpulo. Plantas de cobertura para o cultivo do lúpulo. Manejo da água para a cultura do lúpulo. Artropodofauna de Humulus lupulus L. Manejo e controle fitossanitário da cultura do lúpulo. Colheita e tecnologias pós-colheita para preservação da qualidade de estróbilo ou cone do lúpulo. Perspectivas de Mercado para o lúpulo produzido na região serrana fluminense. |
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Thesagro: |
Lúpulo; Mercado; Sistema de Produção. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144201/1/Manual-de-boas-praticas-de-Lupulo.pdf
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Marc: |
LEADER 01368nam a2200217 a 4500
001 2144201
005 2022-06-21
008 2022 bl uuuu 00u1 u #d
020 $a978-65-992575-1-3
100 1 $aAQUINO, A. M. de
245 $aProdução de lúpulo na Região Serrana Fluminense$bmanual de boas práticas.$h[electronic resource]
260 $aNova Friburgo: Associação Comercial, Industrial e Agrícola de Nova Friburgo - ACIANF$c2022
300 $a140 p.
520 $aRede para o fomento à cultura do lúpulo na região serrana fluminense. Zoneamento Agroecológico preliminar para a cultura do lúpulo no Estado do Rio de Janeiro. Mudas de Lúpulo (Humulus lupulus L.). Manejo e condução da cultura do lúpulo. Recomendações preliminares de calagem e adubação para a cultura do lúpulo. Plantas de cobertura para o cultivo do lúpulo. Manejo da água para a cultura do lúpulo. Artropodofauna de Humulus lupulus L. Manejo e controle fitossanitário da cultura do lúpulo. Colheita e tecnologias pós-colheita para preservação da qualidade de estróbilo ou cone do lúpulo. Perspectivas de Mercado para o lúpulo produzido na região serrana fluminense.
650 $aLúpulo
650 $aMercado
650 $aSistema de Produção
700 1 $aTEIXEIRA, A. J.
700 1 $aFONSECA, M. J. de O.
700 1 $aASSIS, R. L. de
700 1 $aOZASSA, T. I.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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