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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
17/11/2021 |
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Data da última atualização: |
17/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, J. D. R.; BATISTA, R. I. T. P.; UNGERFELD, R.; TAIRA, A. R.; ESPÍRITO SANTO, C. G. do; SOUZA-FABJAN, J. M. G.; FERNANDES, D. A. de M.; BALARO, M. F. A.; COSENTINO, I. O.; BRAIR, V. L.; PINTO, P. H. N.; CARVALHO, A. B. da S.; FONSECA, J. F. da; BRANDÃO, F. Z. |
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Afiliação: |
JULIANA DANTAS RODRIGUES SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; RODOLFO UNGERFELD, Universidad de la República - Montevideo, Uruguay; AUGUSTO RYONOSUKE TAIRA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; CAROLINE GOMES DO ESPÍRITO SANTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JOANNA MARIA GONÇALVES SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; DANIEL ANDREWS DE MOURA FERNANDES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ISABEL OLIVEIRA COSENTINO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; VIVIANE LOPES BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; PEDRO HENRIQUE NICOLAU PINTO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; ANA BEATRIZ DA SILVA CARVALHO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil.; JEFERSON FERREIRA DA FONSECA, CNPC; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
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Título: |
Hormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Record, e1064, 2021. |
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DOI: |
https://doi.org/10.1002/vetr.1064 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis. MenosAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the eff... Mostrar Tudo |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02718naa a2200301 a 4500
001 2136205
005 2021-11-17
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/vetr.1064$2DOI
100 1 $aSANTOS, J. D. R.
245 $aHormonal protocol used for cervical dilation in ewes does not affect morphological embryo quality but reduces recovery rate and temporarily alters gene expression.$h[electronic resource]
260 $c2021
520 $aAbstract: Background: Information on the impact of hormonal protocols for cervical dilation on the quality of ovine embryos is scarce. Methods: To compare the quality of embryos after cervical dilation protocol, ewes (n = 64) were allocated into either a treated group (100 ?g estradiol benzoate intravenous and 0.12 mg cloprostenol intramuscularly, 12 hours before embryo collection plus 100 iu oxytocin intravenous 15 minutes before the collection procedure) or a control group (saline). Luteal function was analysed using ultrasonography and P4 measurement. Some collected embryos were frozen/thawed for gene expression, others were cultured in vitro, frozen/thawed for gene expression, and the remaining embryos were fixed for the apoptosis test (TUNEL test). Results: The treatment reduced fluid (p=0.04) and structure (p=0.03) recovery rates, but the morphological quality, development stage, and apoptosis incidence of the embryos were not affected by treatment. The corpora lutea of the control group had greater blood perfusion (p = 0.002) and greater P4 concentrations at 6, 9, and 12 h after the treatment (p < 0.0001). The expression of BAX, BCL2, PRDX1, and HSP90 genes were not affected by the treatment. However, the embryos in the treated group had fewer NANOG and OCT4 transcripts than control embryos (p = 0.008; p = 0.006, respectively). After culture, there was no difference between the groups in any gene. Conclusion: The hormonal protocol for cervical dilation reduced the efficiency of embryo collection. In addition, the treatment induced luteolysis and a transient alteration of embryo gene expression, however there were no detectable changes in embryo morphological quality, development stage, or incidence of apoptosis.
650 $aSheep
700 1 $aBATISTA, R. I. T. P.
700 1 $aUNGERFELD, R.
700 1 $aTAIRA, A. R.
700 1 $aESPÍRITO SANTO, C. G. do
700 1 $aSOUZA-FABJAN, J. M. G.
700 1 $aFERNANDES, D. A. de M.
700 1 $aBALARO, M. F. A.
700 1 $aCOSENTINO, I. O.
700 1 $aBRAIR, V. L.
700 1 $aPINTO, P. H. N.
700 1 $aCARVALHO, A. B. da S.
700 1 $aFONSECA, J. F. da
700 1 $aBRANDÃO, F. Z.
773 $tVeterinary Record, e1064, 2021.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
16/02/2022 |
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Data da última atualização: |
22/02/2022 |
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Autoria: |
CARVALHO, P. E. R. |
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Afiliação: |
PAULO ERNANI RAMALHO CARVALHO, CNPF. |
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Título: |
Guapuruvu: Schizolobium parahybae. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: CARVALHO, P. E. R. Espécies arbóreas brasileiras. Brasília: Embrapa Informação Tecnológica; Colombo: Embrapa Florestas, 2003. p. 495-503. |
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Série: |
(Coleção espécies arbóreas brasileiras, v. 1). |
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Idioma: |
Português |
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Conteúdo: |
NOMES VULGARES NO BRASIL: baageiro, bacuru, bageiro, ficha, guapiruvu e pau-de-canoa, no Estado de São Paulo; bacumbu; bacuparu; bacurubu, na Bahia, no Estado do Rio de Janeiro, em Santa Catarina e no Estado de São Paulo; bacurubu-ficheira; bacuruva; bacuruvu, gapuruvu, garapuvu e igarapobu, em Santa Catarina; bandarra e guapururru, no Estado do Rio de Janeiro; beri; birosca, breu e guaperuvu, em Minas Gerais; bocurubu; bucuruva; espanador-do-céu; fava-divina, nos Estados do Rio de Janeiro e de São Paulo; faveira; faveiro; ficheira, na Bahia e no Estado de São Paulo; ficheiro; gabiruvu; garapuva; guaburuvu; guaperubu; guapivuçu; guaporuva; guapububu; guapurubu; guarapuvu; guavirovo, no Rio Grande do Sul; paricá; pataqueira; pau-vintém; pau-de-tambor; pau-de-vintém, pinho e pino, na Bahia; pinho-branco; e pirosca. |
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Palavras-Chave: |
Descrição; Ocorrência; Schizolobium parahybae; Uso. |
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Thesagro: |
Crescimento; Espécie Nativa; Guapuruvu; Madeira; Nomenclatura; Taxonomia. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/231708/1/Especies-Arboreas-Brasileiras-vol-1-Guapuruvu.pdf
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Marc: |
LEADER 01590naa a2200253 a 4500
001 2140131
005 2022-02-22
008 2003 bl uuuu u00u1 u #d
100 1 $aCARVALHO, P. E. R.
245 $aGuapuruvu$bSchizolobium parahybae.
260 $c2003
490 $a(Coleção espécies arbóreas brasileiras, v. 1).
520 $aNOMES VULGARES NO BRASIL: baageiro, bacuru, bageiro, ficha, guapiruvu e pau-de-canoa, no Estado de São Paulo; bacumbu; bacuparu; bacurubu, na Bahia, no Estado do Rio de Janeiro, em Santa Catarina e no Estado de São Paulo; bacurubu-ficheira; bacuruva; bacuruvu, gapuruvu, garapuvu e igarapobu, em Santa Catarina; bandarra e guapururru, no Estado do Rio de Janeiro; beri; birosca, breu e guaperuvu, em Minas Gerais; bocurubu; bucuruva; espanador-do-céu; fava-divina, nos Estados do Rio de Janeiro e de São Paulo; faveira; faveiro; ficheira, na Bahia e no Estado de São Paulo; ficheiro; gabiruvu; garapuva; guaburuvu; guaperubu; guapivuçu; guaporuva; guapububu; guapurubu; guarapuvu; guavirovo, no Rio Grande do Sul; paricá; pataqueira; pau-vintém; pau-de-tambor; pau-de-vintém, pinho e pino, na Bahia; pinho-branco; e pirosca.
650 $aCrescimento
650 $aEspécie Nativa
650 $aGuapuruvu
650 $aMadeira
650 $aNomenclatura
650 $aTaxonomia
653 $aDescrição
653 $aOcorrência
653 $aSchizolobium parahybae
653 $aUso
773 $tIn: CARVALHO, P. E. R. Espécies arbóreas brasileiras. Brasília: Embrapa Informação Tecnológica; Colombo: Embrapa Florestas, 2003. p. 495-503.
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