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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
20/11/2007 |
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Data da última atualização: |
24/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MÉO, S. C.; YAMAZAKI, W.; FERREIRA, C. R.; PERECIN, F.; SARAIVA, N. Z.; LEAL, C. L. V.; GARCIA, J. M. |
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Afiliação: |
SIMONE CRISTINA MEO, CPPSE; W. YAMAZAKI, Unesp/Jaboticabal; C. R. FERREIRA, Unesp/Jaboticabal; F. PERECIN, Unesp/Jaboticabal; N. Z. SARAIVA, Unesp/ Jaboticabal; C. L. V. LEAL, FZEA/USP; J. M. GARCIA, Unesp/Jaboticabal. |
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Título: |
Parthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Zygote, v. 15, p. 295-306, nov. 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of oocyte age on early parthenogenesis was assessed in oocytes IVM for 22, 26 and 30 h. Better results in pronuclear development were obtained in treatments ISD (81.7%) at 22 h; D (66.7%), IS (63.3%), ID (73.3%) and ISD (76.7%) at 26 h; and D (86.7%), IS (85.0%) and ID (78.3%) at 30 h. Higher cleavage occurred on ISD (80.0%) at 22 h; ID (83.3%) and ISD (91.7%) at 26 h; and I (86.7%), IS (90.0%), ID (85.0%) and ISD (95.0%) at 30 h. More blastocysts were achieved in ID (25.0%) and ISD (18.3%) at 22 h; and in ID at 26 h (45.0%) and 30 h (50.0%). We also observed that IS allowed higher haploid (77.4%) embryonic development, whilst ID was better for diploid (89.1%) development. It was concluded that association of S and D without I was not effective for blastocyst development; treatments using S were less influenced by oocyte age, but when S was associated with D here was a detrimental effect on aged oocytes; treatment ISD promoted higher activation and cleavage rates in young oocytes and ID protocol was the best for producing blastocysts. |
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Palavras-Chave: |
Embryonic development; Oocyte activation. |
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Thesaurus Nal: |
cattle; chromatin; microtubules. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02150naa a2200253 a 4500
001 1048149
005 2023-02-24
008 2007 bl uuuu u00u1 u #d
100 1 $aMÉO, S. C.
245 $aParthenogenetic activation of bovine oocytes using single and combined strontium, ionomycin and 6-dimethylaminopurine treatments.$h[electronic resource]
260 $c2007
520 $aIn vitro-matured (IVM) bovine oocytes were activated with single and combined treatments of strontium (S), ionomycin (I) and 6-DMAP (D). Using oocytes IVM for 26 h, we observed that activation altered cell cycle kinetics (faster progression, MIII arrest, or direct transition from MII to pronuclear stage) when compared to in vitro fertilization. The effect of oocyte age on early parthenogenesis was assessed in oocytes IVM for 22, 26 and 30 h. Better results in pronuclear development were obtained in treatments ISD (81.7%) at 22 h; D (66.7%), IS (63.3%), ID (73.3%) and ISD (76.7%) at 26 h; and D (86.7%), IS (85.0%) and ID (78.3%) at 30 h. Higher cleavage occurred on ISD (80.0%) at 22 h; ID (83.3%) and ISD (91.7%) at 26 h; and I (86.7%), IS (90.0%), ID (85.0%) and ISD (95.0%) at 30 h. More blastocysts were achieved in ID (25.0%) and ISD (18.3%) at 22 h; and in ID at 26 h (45.0%) and 30 h (50.0%). We also observed that IS allowed higher haploid (77.4%) embryonic development, whilst ID was better for diploid (89.1%) development. It was concluded that association of S and D without I was not effective for blastocyst development; treatments using S were less influenced by oocyte age, but when S was associated with D here was a detrimental effect on aged oocytes; treatment ISD promoted higher activation and cleavage rates in young oocytes and ID protocol was the best for producing blastocysts.
650 $acattle
650 $achromatin
650 $amicrotubules
653 $aEmbryonic development
653 $aOocyte activation
700 1 $aYAMAZAKI, W.
700 1 $aFERREIRA, C. R.
700 1 $aPERECIN, F.
700 1 $aSARAIVA, N. Z.
700 1 $aLEAL, C. L. V.
700 1 $aGARCIA, J. M.
773 $tZygote$gv. 15, p. 295-306, nov. 2007.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
13/11/2020 |
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Data da última atualização: |
13/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
FÉLIX, D. O.; SILVA, A. S.; FIALHO, A. T. S.; OLIVEIRA, C. J. P; BRITO, C. O.; TAVERNARI, F. de C.; RIBEIRO JUNIOR, V.; ROSTAGNO, H. S.; ALBINO, L. F. T. |
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Afiliação: |
UFV; UFS; UFS; UFS; CLAUDSON OLIVEIRA BRITO, UFS; FERNANDO DE CASTRO TAVERNARI, CNPSA; VALDIR RIBEIRO JUNIOR, UFS; HORÁCIO SANTIAGO ROSTAGNO, UFV; LUIZ FERNANDO TEIXEIRA ALBINO, UFV. |
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Título: |
Fat-soluble vitamin supplementation levels in diets for laying hens from 28 to 44 weeks of age. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Poultry Science, v. 22, n. 3, p. 1-10, 2020. |
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DOI: |
http://dx.doi.org/10.1590/1806-9061-2019-1239 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstratc: This study aimed to examine the impact of levels of dietary supplementation with fat-soluble vitamins on the production performance and egg quality of laying hens. Three hundred Hy-Line White W-36 laying hens were evaluated from 28 to 44 weeks of age. The birds were allotted to one of six treatments in a randomized block design with 10 replicates with five birds each. Performance and egg quality parameters were evaluated in four 28-day periods. A corn and soybean meal-based basal diet was formulated so as to meet the nutritional requirements of the animals, with the exception of fatsoluble vitamins. The treatments consisted of dietary supplementation with 0%, 33.3%, 66.7%, 100.0%, 133.3% or 166.7% of fat-soluble vitamins (100% supplementation consisted of 7500 IU, 2000 IU, 10 IU and 1.8 mg of vitamins A, D3, E and K per kilogram of diet, respectively). Eggshell weight, shell thickness, shell strength, feed intake, egg weight, feed conversion per egg mass and feed conversion per dozen eggs showed a quadratic response (p≤0.05) to the treatments, whereas egg mass responded linearly. Optimal results were obtained at an average fat-soluble vitamin supplementation level of 109%, which corresponds to 8175 IU of vitamin A, 2180 IU of vitamin D3, 10.9 IU of vitamin E and 1.96 mg of vitamin K per kilogram of diet. |
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Palavras-Chave: |
Casca do ovo; Peso do ovo; Poedeira; Suplementação de vitaminas; White eggs. |
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Thesagro: |
Dieta; Performance. |
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Thesaurus NAL: |
Egg weight. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/217768/1/final9539.pdf
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Marc: |
LEADER 02307naa a2200325 a 4500
001 2126555
005 2020-11-13
008 2020 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/1806-9061-2019-1239$2DOI
100 1 $aFÉLIX, D. O.
245 $aFat-soluble vitamin supplementation levels in diets for laying hens from 28 to 44 weeks of age.$h[electronic resource]
260 $c2020
520 $aAbstratc: This study aimed to examine the impact of levels of dietary supplementation with fat-soluble vitamins on the production performance and egg quality of laying hens. Three hundred Hy-Line White W-36 laying hens were evaluated from 28 to 44 weeks of age. The birds were allotted to one of six treatments in a randomized block design with 10 replicates with five birds each. Performance and egg quality parameters were evaluated in four 28-day periods. A corn and soybean meal-based basal diet was formulated so as to meet the nutritional requirements of the animals, with the exception of fatsoluble vitamins. The treatments consisted of dietary supplementation with 0%, 33.3%, 66.7%, 100.0%, 133.3% or 166.7% of fat-soluble vitamins (100% supplementation consisted of 7500 IU, 2000 IU, 10 IU and 1.8 mg of vitamins A, D3, E and K per kilogram of diet, respectively). Eggshell weight, shell thickness, shell strength, feed intake, egg weight, feed conversion per egg mass and feed conversion per dozen eggs showed a quadratic response (p≤0.05) to the treatments, whereas egg mass responded linearly. Optimal results were obtained at an average fat-soluble vitamin supplementation level of 109%, which corresponds to 8175 IU of vitamin A, 2180 IU of vitamin D3, 10.9 IU of vitamin E and 1.96 mg of vitamin K per kilogram of diet.
650 $aEgg weight
650 $aDieta
650 $aPerformance
653 $aCasca do ovo
653 $aPeso do ovo
653 $aPoedeira
653 $aSuplementação de vitaminas
653 $aWhite eggs
700 1 $aSILVA, A. S.
700 1 $aFIALHO, A. T. S.
700 1 $aOLIVEIRA, C. J. P
700 1 $aBRITO, C. O.
700 1 $aTAVERNARI, F. de C.
700 1 $aRIBEIRO JUNIOR, V.
700 1 $aROSTAGNO, H. S.
700 1 $aALBINO, L. F. T.
773 $tBrazilian Journal of Poultry Science$gv. 22, n. 3, p. 1-10, 2020.
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Embrapa Suínos e Aves (CNPSA) |
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