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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
04/11/2011 |
Data da última atualização: |
19/09/2013 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
MARTINS, L. A. R.; SILVA, E. P.; PRAÇA, T. M.; LAVIOLA, B. G.; PRAÇA-FONTES, M. M. |
Afiliação: |
L. A. R. MARTINS, UFES; E. P. SILVA, UFES; T. M. PRAÇA, UFV; BRUNO GALVEAS LAVIOLA, CNPAE; M. M. PRAÇA-FONTES, UFES. |
Título: |
Análise da viabilidade dos grão de pólen de diferentes acessos de pinhão manso (Jatropha curcas). |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: ENCONTRO LATINO AMERICANO DE INICIAÇÃO CIENTÍFICA, 16.; ENCONTRO LATINO AMERICANO DE PÓS GRADUAÇÃO, 11., 2011, São José dos Campos, SP. As contribuições da ciência para a sustentabilidade do planeta. São José dos Campos: UNIVAP, 2011. |
Idioma: |
Português |
Palavras-Chave: |
Lugol; Orceína acética; Viabilidade polínica. |
Thesagro: |
Jatropha Curcas. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00794nam a2200193 a 4500 001 1904993 005 2013-09-19 008 2011 bl uuuu u00u1 u #d 100 1 $aMARTINS, L. A. R. 245 $aAnálise da viabilidade dos grão de pólen de diferentes acessos de pinhão manso (Jatropha curcas). 260 $aIn: ENCONTRO LATINO AMERICANO DE INICIAÇÃO CIENTÍFICA, 16.; ENCONTRO LATINO AMERICANO DE PÓS GRADUAÇÃO, 11., 2011, São José dos Campos, SP. As contribuições da ciência para a sustentabilidade do planeta. São José dos Campos: UNIVAP$c2011 650 $aJatropha Curcas 653 $aLugol 653 $aOrceína acética 653 $aViabilidade polínica 700 1 $aSILVA, E. P. 700 1 $aPRAÇA, T. M. 700 1 $aLAVIOLA, B. G. 700 1 $aPRAÇA-FONTES, M. M.
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
Data corrente: |
01/09/2020 |
Data da última atualização: |
19/11/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 5 |
Autoria: |
RIBEIRO, T. P.; BASSO, M. F.; CARVALHO, M. H. de; MACEDO, L. L. P. de; SILVA, D. M. L. da; LOURENCO-TESSUTTI, I. T.; OLIVEIRA-NETO, O. B. de; CAMPOS-PINTO, E. R. de; LUCENA, W. A.; SILVA, M. C. M. da; TRIPODE, B. M. D.; ABREU-JARDIM, T. P. F.; MIRANDA, J. E.; ALVES-FERREIRA, M.; MORGANTE, C. V.; GROSSI-DE-SA, M. F. |
Afiliação: |
THUANNE PIRES RIBEIRO, UNB; MARCOS FERNANDO BASSO; MAYARA HOLANDA DE CARVALHO; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; DAGNA MARIA LAURINDO DA SILVA; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; OSMUNDO BRILANTE DE OLIVEIRA-NETO; EDUARDO ROMANO DE CAMPOS PINTO, Cenargen; WAGNER ALEXANDRE LUCENA, Cenargen; MARIA CRISTINA MATTAR DA SILVA, Cenargen; BRUNA MENDES DINIZ TRIPODE, CNPA; TATIANNE PIZA FERRARI ABREU-JARDIM; JOSE EDNILSON MIRANDA, CNPA; MARCIO ALVES-FERREIRA, UFRJ; CAROLINA VIANNA MORGANTE, CPATSA; MARIA FATIMA GROSSI DE SA, Cenargen. |
Título: |
Stability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Biotechnology Research and Innovation, v. 3, p. 27-41, 2020. |
DOI: |
https://doi.org/10.1016/j.biori.2019.12.003 |
Idioma: |
Inglês |
Conteúdo: |
The cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW. MenosThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events ... Mostrar Tudo |
Palavras-Chave: |
Bacillus; Bicudo do algodão; Entomotoxin; Herança estável; Insect pest; Management; Praga de inseto; Resistance; Resistência gestão; Stable inheritance; Thuringiensis. |
Thesagro: |
Algodão; Inseto; Praga; Praga de Planta. |
Thesaurus NAL: |
Cotton; Insect pests; Resistance management. |
Categoria do assunto: |
-- O Insetos e Entomologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/216823/1/Stability-and-tissue-2020.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/215669/1/1-s2.0-S2452072119301352-main.pdf
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Marc: |
LEADER 03317naa a2200529 a 4500 001 2125667 005 2020-11-19 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.biori.2019.12.003$2DOI 100 1 $aRIBEIRO, T. P. 245 $aStability and tissue-specific Cry10Aa overexpression improves cotton resistance to the cotton boll weevil.$h[electronic resource] 260 $c2020 520 $aThe cotton boll weevil (CBW, Anthonomus grandis) is the most destructive cotton insect pest affecting cotton crops. To overcome this problem, CBW-resistant genetically modified cotton plants overexpressing Bacillus thuringiensis entomotoxins were successfully obtained. Previous results showed that the overexpression of Cry10Aa protoxin led to high mortality of the CBW larvae in greenhouse conditions. In this study, we advanced three more generations (T2 to T4), with several cotton events constitutively overexpressing the Cry10Aa protoxin, and the transgene stability and agronomic performance were investigated. In addition, stable transgenic cotton overexpressing the Cry10Aa active (Cry10Aa protoxin lacking the -helix N-terminal) driven by cotton flower bud-specific promoters were generated and characterized. Cotton events constitutively or tissue-specifically overexpressing the Cry10Aa protein (protoxin or active) represented mortality percentages of the CBW larva of up to 85 % in plants under greenhouse conditions. Events overexpressing the Cry10Aa active under control of the flower bud-specific promoter showed higher protein accumulation in stamens and carpels compared to the events with constitutive expression. Our findings suggested that the high stability of the Cry10Aa transgene and the elevated expression level and protein accumulation in flower bud tissues (primarily in stamen and carpels) contribute to improved resistance to CBW larvae. Finally, some notable events were selected with potential for future field trials in different cotton- producing regions of Brazil. Therefore, cotton events overexpressing high levels of the Cry10Aa protein in flower bud tissue may have a strong potential for commercial use in the integrated management of CBW. 650 $aCotton 650 $aInsect pests 650 $aResistance management 650 $aAlgodão 650 $aInseto 650 $aPraga 650 $aPraga de Planta 653 $aBacillus 653 $aBicudo do algodão 653 $aEntomotoxin 653 $aHerança estável 653 $aInsect pest 653 $aManagement 653 $aPraga de inseto 653 $aResistance 653 $aResistência gestão 653 $aStable inheritance 653 $aThuringiensis 700 1 $aBASSO, M. F. 700 1 $aCARVALHO, M. H. de 700 1 $aMACEDO, L. L. P. de 700 1 $aSILVA, D. M. L. da 700 1 $aLOURENCO-TESSUTTI, I. T. 700 1 $aOLIVEIRA-NETO, O. B. de 700 1 $aCAMPOS-PINTO, E. R. de 700 1 $aLUCENA, W. A. 700 1 $aSILVA, M. C. M. da 700 1 $aTRIPODE, B. M. D. 700 1 $aABREU-JARDIM, T. P. F. 700 1 $aMIRANDA, J. E. 700 1 $aALVES-FERREIRA, M. 700 1 $aMORGANTE, C. V. 700 1 $aGROSSI-DE-SA, M. F. 773 $tBiotechnology Research and Innovation$gv. 3, p. 27-41, 2020.
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