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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
03/05/2021 |
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Data da última atualização: |
03/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MATHIOUDAKIS, M. M.; MALIOGKA, V. I.; CANDRESSE, T.; NICKEL, O.; FAJARDO, T. V. M.; BUDZYNSKA, D.; JAROSZEWSKA, B. H.; KATIS, N. I. |
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Afiliação: |
MATTHAIOS M. MATHIOUDAKIS, Plant Pathology Laboratory, Institute of Olive Tree, Subtropical Plants & Viticulture, ELGO-DIMITRA, Karamanlis Ave. 167, Gr-73134 Chania, Greece; VARVARA I. MALIOGKA, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Gr-54124 Thessaloniki, Greece; vmaliogk@agro.auth.gr (V.I.M.); katis@agro.auth.gr (N.I.K.); THIERRY CANDRESSE, INRAE, UMR BFP, University of Bordeaux, CS20032, 33882 Villenave d’Ornon CEDEX, France; thierry.candresse@inrae.fr; OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV; DARIA BUDZYNSKA, Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul. W?. W?egorka 20, 60-318 Pozna ´n, Poland; d.budzynska@iorpib.poznan.pl (D.B.); b.hasiow@iorpib.poznan.pl (B.H.-J.) * Correspondence: mathioudakis@elgo.; BEATA HASIOW JAROSZEWSKA, Department of Virology and Bacteriology, Institute of Plant Protection-National Research Institute, ul.; NIKILAOS I. KATIS, Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, Gr-54124 Thessaloniki, Greece. |
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Título: |
Molecular characterization of the coat protein gene of greek apple stem pitting virus isolates: evolution through deletions, insertions, and recombination events. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plants, v. 10, p. 1-19, e10050917, abril 2021. |
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Páginas: |
19 |
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DOI: |
10.3390/plants10050917 |
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Idioma: |
Inglês |
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Conteúdo: |
A RT-PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which represent hallmarks of specific lineages and indicate their possible role in virus diversification, rather than the CP size itself. Evidence of recombination between isolates from botanical and cultivated species and the clustering of isolates from botanical species and isolates from cultivated species suggest the existence of a possible undetermined transmission mechanism allowing the exchange of ASPV isolates between the cultivated and wild/ornamental hosts. MenosA RT-PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which ... Mostrar Tudo |
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Palavras-Chave: |
ASPV. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/223000/1/plants-10-00917.pdf
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Marc: |
LEADER 02725naa a2200241 a 4500
001 2131631
005 2021-05-03
008 2021 bl uuuu u00u1 u #d
024 7 $a10.3390/plants10050917$2DOI
100 1 $aMATHIOUDAKIS, M. M.
245 $aMolecular characterization of the coat protein gene of greek apple stem pitting virus isolates$bevolution through deletions, insertions, and recombination events.$h[electronic resource]
260 $c2021
300 $a19
520 $aA RT-PCR assay developed to amplify the full coat protein (CP) gene of apple stem pitting virus (ASPV) was evaluated using 180 Greek apple and pear samples and showed a broad detection range. This method was used to investigate the presence of ASPV in quince in Greece and showed a high incidence of 52%. The sequences of 14 isolates from various hosts with a distinct RFLP profile were determined. ASPV population genetics and the factors driving ASPV evolution were analyzed using the Greek ASPV sequences, novel sequences from Brazilian apple trees and Chinese botanical Pyrus species, and homologous sequences retrieved from GenBank. Fourteen variant types of Greek, Brazilian and botanical isolates, which differ in CP gene length and presence of indels, were identified. In addition, these analyses showed high intra- and inter-group variation among isolates from different countries and hosts, indicating the significant variability present in ASPV. Recombination events were detected in four isolates originating from Greek pear and quince and two from Brazilian apples. In a phylogenetic analysis, there was a tendency for isolates to cluster together based on CP gene length, the isolation host, and the detection method applied. Although there was no strict clustering based on geographical origin, most isolates from a given country tended to regroup in specific clusters. Interestingly, it was found that the phylogeny was correlated to the type, position, and pattern of indels, which represent hallmarks of specific lineages and indicate their possible role in virus diversification, rather than the CP size itself. Evidence of recombination between isolates from botanical and cultivated species and the clustering of isolates from botanical species and isolates from cultivated species suggest the existence of a possible undetermined transmission mechanism allowing the exchange of ASPV isolates between the cultivated and wild/ornamental hosts.
653 $aASPV
700 1 $aMALIOGKA, V. I.
700 1 $aCANDRESSE, T.
700 1 $aNICKEL, O.
700 1 $aFAJARDO, T. V. M.
700 1 $aBUDZYNSKA, D.
700 1 $aJAROSZEWSKA, B. H.
700 1 $aKATIS, N. I.
773 $tPlants$gv. 10, p. 1-19, e10050917, abril 2021.
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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Biblioteca(s): |
Embrapa Agrossilvipastoril; Embrapa Solos. |
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Data corrente: |
04/06/2020 |
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Data da última atualização: |
09/07/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NASCIMENTO, A. F. do; RODRIGUES, R. de A. R.; SILVEIRA, J. G. da; SILVA, J. J. N. da; DANIEL, V. de C.; SEGATTO, E. R. |
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Afiliação: |
ALEXANDRE FERREIRA DO NASCIMENTO, CPAMT; RENATO DE ARAGAO RIBEIRO RODRIGUES, CNPS; JULIA GRAZIELA DA SILVEIRA, UFV; JACQUELINE JESUS NOGUEIRA DA SILVA, UFF; VAGNER DE CARVALHO DANIEL, UFMT; EDUARDO RECKERS SEGATTO, UFMT. |
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Título: |
Nitrous oxide emissions from a tropical Oxisol under monocultures and an integrated system in the Southern Amazon - Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência do Solo, v. 44, e0190123, 2020. |
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ISSN: |
1806-9657 |
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DOI: |
https://doi.org/10.36783/18069657rbcs2019 |
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Idioma: |
Inglês |
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Conteúdo: |
Although agriculture and livestock systems represent important sources of N2O from the soil, they may also aid in emissions mitigation, mainly when integrated systems are taken into account, such as crop-livestock-forest, for food production. This work assessed the soil N2O emissions from a tropical Oxisol under row-crop, livestock, forest monocultures, and an integrated crop-livestock-forest system in the Southern Amazon - Brazil. Soil N2O emissions were measured using static chambers from November 2014 to October 2016 in four soil use systems [row-crop, livestock, forest, and integrated crop-livestock-forest (CLF)], and in a reference area under native forest fragment. For the whole period, the average of soil N2O fluxes was 16.9, 12.2, and 15.4 ug N2O-N m-2 h-1, to row-crop, livestock, and CLF systems, respectively, all with a similar average among them. The lowest fluxes were observed in the forest system and native forest fragment, with average fluxes of 4.0 and 6.3 ug N2O-N m-2 h-1, respectively, both lower than the agricultural systems. The largest soil N2O fluxes were observed throughout the rainy seasons in the row-crop, livestock, and CLF, mostly after N-fertilizer application to the soil surface or in the planted row. As a consequence, the cumulative emissions were greater in row-crop, livestock, and CLF systems, which in the averages of two cycles emitted respectively 1.40, 1.15, and 1.27 kg N2O-N ha-1 yr-1, all different of the forest system and native forest fragment (0.33 and 0.52 kg N2O-N ha-1 yr-1, respectively). Nitrogen fertilization and soil moisture influenced soil N2O emissions of all systems assessed in the Southern Amazon. The N2O emissions took place after both factors were met, corroborating the hole-in-the-pipe model. Even with more soil use intensification, once in the same area there were three cultures in succession during a year and perennial trees, CLF did not lead to greater N2O emissions from the soil than row-crop and livestock. Thus, CLF represents a good option for N2O mitigation for the edaphic and climatic conditions of the Southern Amazon. MenosAlthough agriculture and livestock systems represent important sources of N2O from the soil, they may also aid in emissions mitigation, mainly when integrated systems are taken into account, such as crop-livestock-forest, for food production. This work assessed the soil N2O emissions from a tropical Oxisol under row-crop, livestock, forest monocultures, and an integrated crop-livestock-forest system in the Southern Amazon - Brazil. Soil N2O emissions were measured using static chambers from November 2014 to October 2016 in four soil use systems [row-crop, livestock, forest, and integrated crop-livestock-forest (CLF)], and in a reference area under native forest fragment. For the whole period, the average of soil N2O fluxes was 16.9, 12.2, and 15.4 ug N2O-N m-2 h-1, to row-crop, livestock, and CLF systems, respectively, all with a similar average among them. The lowest fluxes were observed in the forest system and native forest fragment, with average fluxes of 4.0 and 6.3 ug N2O-N m-2 h-1, respectively, both lower than the agricultural systems. The largest soil N2O fluxes were observed throughout the rainy seasons in the row-crop, livestock, and CLF, mostly after N-fertilizer application to the soil surface or in the planted row. As a consequence, the cumulative emissions were greater in row-crop, livestock, and CLF systems, which in the averages of two cycles emitted respectively 1.40, 1.15, and 1.27 kg N2O-N ha-1 yr-1, all different of the forest system and native forest fr... Mostrar Tudo |
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Palavras-Chave: |
Mato Grosso; Mitigação; Sinop-MT. |
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Thesagro: |
Aquecimento; Efeito Estufa; Gás; Latossolo; Oxisol; Solo Florestal. |
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Thesaurus NAL: |
Forest soils; Global warming; Greenhouse effect; Greenhouse gases; Oxisols. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214512/1/2020-cpamt-afn-nitrous-emissions-tropical-oxisol-monocultre-integrated-system-sourthen-amazon-brazil.pdf
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Marc: |
LEADER 03207naa a2200373 a 4500
001 2123759
005 2020-07-09
008 2020 bl uuuu u00u1 u #d
022 $a1806-9657
024 7 $ahttps://doi.org/10.36783/18069657rbcs2019$2DOI
100 1 $aNASCIMENTO, A. F. do
245 $aNitrous oxide emissions from a tropical Oxisol under monocultures and an integrated system in the Southern Amazon - Brazil.$h[electronic resource]
260 $c2020
520 $aAlthough agriculture and livestock systems represent important sources of N2O from the soil, they may also aid in emissions mitigation, mainly when integrated systems are taken into account, such as crop-livestock-forest, for food production. This work assessed the soil N2O emissions from a tropical Oxisol under row-crop, livestock, forest monocultures, and an integrated crop-livestock-forest system in the Southern Amazon - Brazil. Soil N2O emissions were measured using static chambers from November 2014 to October 2016 in four soil use systems [row-crop, livestock, forest, and integrated crop-livestock-forest (CLF)], and in a reference area under native forest fragment. For the whole period, the average of soil N2O fluxes was 16.9, 12.2, and 15.4 ug N2O-N m-2 h-1, to row-crop, livestock, and CLF systems, respectively, all with a similar average among them. The lowest fluxes were observed in the forest system and native forest fragment, with average fluxes of 4.0 and 6.3 ug N2O-N m-2 h-1, respectively, both lower than the agricultural systems. The largest soil N2O fluxes were observed throughout the rainy seasons in the row-crop, livestock, and CLF, mostly after N-fertilizer application to the soil surface or in the planted row. As a consequence, the cumulative emissions were greater in row-crop, livestock, and CLF systems, which in the averages of two cycles emitted respectively 1.40, 1.15, and 1.27 kg N2O-N ha-1 yr-1, all different of the forest system and native forest fragment (0.33 and 0.52 kg N2O-N ha-1 yr-1, respectively). Nitrogen fertilization and soil moisture influenced soil N2O emissions of all systems assessed in the Southern Amazon. The N2O emissions took place after both factors were met, corroborating the hole-in-the-pipe model. Even with more soil use intensification, once in the same area there were three cultures in succession during a year and perennial trees, CLF did not lead to greater N2O emissions from the soil than row-crop and livestock. Thus, CLF represents a good option for N2O mitigation for the edaphic and climatic conditions of the Southern Amazon.
650 $aForest soils
650 $aGlobal warming
650 $aGreenhouse effect
650 $aGreenhouse gases
650 $aOxisols
650 $aAquecimento
650 $aEfeito Estufa
650 $aGás
650 $aLatossolo
650 $aOxisol
650 $aSolo Florestal
653 $aMato Grosso
653 $aMitigação
653 $aSinop-MT
700 1 $aRODRIGUES, R. de A. R.
700 1 $aSILVEIRA, J. G. da
700 1 $aSILVA, J. J. N. da
700 1 $aDANIEL, V. de C.
700 1 $aSEGATTO, E. R.
773 $tRevista Brasileira de Ciência do Solo$gv. 44, e0190123, 2020.
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