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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/10/2020 |
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Data da última atualização: |
19/03/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEIXEIRA, N. C.; WENDLAND, A.; OLIVEIRA, M. I. de S.; BRANDÃO, L. T. D.; SOUZA, T. L. P. O. de; FARIA, J. C. de; DEL PELOSO, M. J.; CORTES, M. V. de C. B. |
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Afiliação: |
NARA CRISTINA TEIXEIRA, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; MAYTHSULENE INACIO DE SOUZA OLIVEIRA, bolsista CNPAF; LIVIA TEIXEIRA DUARTE BRANDAO, CNPAF; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF. |
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Título: |
Rapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Crop Science, v. 60, n. 6, p. 3004-3012, Nov./Dec. 2020. |
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ISSN: |
1435-0653 |
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DOI: |
https://doi.org/10.1002/csc2.20107 |
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Idioma: |
Inglês |
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Conteúdo: |
Amolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry. MenosAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaO... Mostrar Tudo |
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Thesagro: |
DNA; Feijão; Mosaico Dourado; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus Nal: |
Bean golden mosaic virus; Beans; Loop-mediated isothermal amplification; RNA interference; Transgenic plants. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02809naa a2200349 a 4500
001 2125441
005 2021-03-19
008 2020 bl uuuu u00u1 u #d
022 $a1435-0653
024 7 $ahttps://doi.org/10.1002/csc2.20107$2DOI
100 1 $aTEIXEIRA, N. C.
245 $aRapid identification of RNA-interference-based resistance to Bean golden mosaic virus in transgenic common beans via loop-mediated isothermal amplification.$h[electronic resource]
260 $c2020
520 $aAmolecular toolwas adapted for practical identification of theworld?s first transgenic event (Embrapa 5.1) in common bean (Phaseolus vulgaris L.). Based on the technology of RNA interference and on the transformation of plants via the Biobalistic method, a transgenic bean was created with effective resistance to Bean golden mosaic virus, its principal virus. To support the monitoring of this technology, the development of new cultivars, and the process of producing, benefiting, and distributing of seeds and grains, a molecular detection tool based on loop-mediated isothermal amplification (LAMP) was developed. This tool can facilitating diagnosis, making it fast, specific, low-cost, and easily executed. Based on the gene Ahas (GenBank M88686) of the Embrapa 5.1 event, sets of initiators were planned (forward outer primer?backward outer primer and forward inner primer?backward inner primer) that are characteristic of the LAMP method. These were evaluated for their sensitivity and specificity in detecting their target. The LAMP method allows direct visual interpretation. The Neutral Red (NR) pH indicator was adopted to facilitate molecular diagnosis in moderately well equipped environments, thus reducing the risk of contamination and the need for skilled labor. This agility in diagnosis occurs because several phases commonly used inmolecular techniques, such as polymerase chain reaction, can be excluded. The LAMPmethod, combined with a rapid DNA extraction method (modified NaOH) and the addition of NR, provides technological support for addressing issues of biosafety, traceability, and identification of the Embrapa 5.1 event in beans in the field and in industry.
650 $aBean golden mosaic virus
650 $aBeans
650 $aLoop-mediated isothermal amplification
650 $aRNA interference
650 $aTransgenic plants
650 $aDNA
650 $aFeijão
650 $aMosaico Dourado
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
700 1 $aWENDLAND, A.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aBRANDÃO, L. T. D.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aFARIA, J. C. de
700 1 $aDEL PELOSO, M. J.
700 1 $aCORTES, M. V. de C. B.
773 $tCrop Science$gv. 60, n. 6, p. 3004-3012, Nov./Dec. 2020.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
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Cutter |
Registro |
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URL |
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste. |
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Data corrente: |
22/06/2020 |
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Data da última atualização: |
12/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
CRUZ, T. T.; ASMUS, G. L.; GARCIA, R. A. |
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Afiliação: |
THAYS TORQUATO CRUZ, PÓS-GRADUAÇÃO EM AGRONOMIA - UNIVERSIDADE ESTADUAL DE MATO GROSSO DO SUL, AQUIDAUANA, MS; GUILHERME LAFOURCADE ASMUS, CPAO; RODRIGO ARROYO GARCIA, CPAO. |
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Título: |
Crotalaria species in succession to soybean for the management of Pratylenchus brachyurus. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Ciência Rural, v. 50, n. 7, e20190645, 2020. |
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DOI: |
doi.org/10.1590/0103-8478cr20190645 |
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Idioma: |
Inglês |
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Palavras-Chave: |
Crotalaria ochroleuca; Lesão radicular. |
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Thesagro: |
Brachiaria Brizantha; Crotalária; Glycine Max; Milho; Nematóide; Soja; Zea Mays. |
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Categoria do assunto: |
A Sistemas de Cultivo |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/214122/1/37033.pdf
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Marc: |
LEADER 00738naa a2200253 a 4500
001 2123398
005 2020-08-12
008 2020 bl uuuu u00u1 u #d
024 7 $adoi.org/10.1590/0103-8478cr20190645$2DOI
100 1 $aCRUZ, T. T.
245 $aCrotalaria species in succession to soybean for the management of Pratylenchus brachyurus.$h[electronic resource]
260 $c2020
650 $aBrachiaria Brizantha
650 $aCrotalária
650 $aGlycine Max
650 $aMilho
650 $aNematóide
650 $aSoja
650 $aZea Mays
653 $aCrotalaria ochroleuca
653 $aLesão radicular
700 1 $aASMUS, G. L.
700 1 $aGARCIA, R. A.
773 $tCiência Rural$gv. 50, n. 7, e20190645, 2020.
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Embrapa Agropecuária Oeste (CPAO) |
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