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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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Data corrente: |
24/08/2023 |
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Data da última atualização: |
04/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, F. M. da; HAACH, V.; BELLAVER, F. V.; BOMBASSARO, G.; GAVA, D.; SILVA, L. P. da; BARON, L. F.; SIMONELLY, M.; CARVALHO, W. A.; SCHAEFER, R.; BASTOS, A. P. A. |
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Afiliação: |
FRANCISCO NOE DA FONSECA, GEM; VANESSA HAACH, Universidade Federal do Rio Grande do Sul; FRANCIANA VOLPATO BELLAVER, Instituto Federal Catarinense; GABRIELLY BOMBASSARO, Instituto Federal Catarinense; DANIELLE GAVA, CNPSA; LUCIANO PAULINO DA SILVA, Cenargen; LANA FLAVIA BARON, Universidade Federal do Rio Grande do Sul; MAYARA SIMONELLY, Universidade de Brasília; WANESSA ARAUJO CARVALHO, CNPGL; REJANE SCHAEFER, CNPSA; ANA PAULA ALMEIDA BASTOS, CNPSA. |
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Título: |
Immunological profile of mice immunized with a polyvalent virosome-based influenza vaccine. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Virology Journal, v. 20, 2023. Article number 187. |
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DOI: |
https://doi.org/10.1186/s12985-023-02158-0 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Francisco Noé Fonseca; Ana Paula Bastos. |
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Conteúdo: |
Abstract
Background: Influenza A virus (IAV) causes respiratory disease in pigs and is a major concern for public health.
Vaccination of pigs is the most successful measure to mitigate the impact of the disease in the herds. Influenza-based
virosome is an effective immunomodulating carrier that replicates the natural antigen presentation pathway and has
tolerability profile due to their purity and biocompatibility.
Methods: This study aimed to develop a polyvalent virosome influenza vaccine containing the hemagglutinin and
neuraminidase proteins derived from the swine IAVs (swIAVs) H1N1, H1N2 and H3N2 subtypes, and to investigate
its effectiveness in mice as a potential vaccine for swine. Mice were immunized with two vaccine doses (1 and 15
days), intramuscularly and intranasally. At 21 days and eight months later after the second vaccine dose, mice were
euthanized. The humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with a
polyvalent influenza virosomal vaccine were investigated.
Results: Only intramuscular vaccination induced high hemagglutination inhibition (HI) titers. Seroconversion
and seroprotection (>4-fold rise in HI antibody titers, reaching a titer of ?1:40) were achieved in 80% of mice
(intramuscularly vaccinated group) at 21 days after booster immunization. Virus-neutralizing antibody titers against
IAV were detected at 8 months after vaccination, indicating long-lasting immunity. Overall, mice immunized with the
virosome displayed greater ability for B, effector-T and memory-T cells from the spleen to respond to H1N1, H1N2 and
H3N2 antigens.
Conclusions: All findings showed an efficient immune response against IAVs in mice vaccinated with a polyvalent
virosome-based influenza vaccine. MenosAbstract
Background: Influenza A virus (IAV) causes respiratory disease in pigs and is a major concern for public health.
Vaccination of pigs is the most successful measure to mitigate the impact of the disease in the herds. Influenza-based
virosome is an effective immunomodulating carrier that replicates the natural antigen presentation pathway and has
tolerability profile due to their purity and biocompatibility.
Methods: This study aimed to develop a polyvalent virosome influenza vaccine containing the hemagglutinin and
neuraminidase proteins derived from the swine IAVs (swIAVs) H1N1, H1N2 and H3N2 subtypes, and to investigate
its effectiveness in mice as a potential vaccine for swine. Mice were immunized with two vaccine doses (1 and 15
days), intramuscularly and intranasally. At 21 days and eight months later after the second vaccine dose, mice were
euthanized. The humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with a
polyvalent influenza virosomal vaccine were investigated.
Results: Only intramuscular vaccination induced high hemagglutination inhibition (HI) titers. Seroconversion
and seroprotection (>4-fold rise in HI antibody titers, reaching a titer of ?1:40) were achieved in 80% of mice
(intramuscularly vaccinated group) at 21 days after booster immunization. Virus-neutralizing antibody titers against
IAV were detected at 8 months after vaccination, indicating long-lasting immunity. Overall, mice immunized with the
virosome... Mostrar Tudo |
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Palavras-Chave: |
Nanovaccine; Nanovacina; Nanovacinação; Seroprotection; Soro imunológico; Soro proteção; Soroproteção; Vaccine. |
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Thesagro: |
Influenza Aviaria; Vacina; Vacinação; Vírus. |
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Thesaurus Nal: |
Influenza A virus; Vaccination. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156329/1/Immunological-profile-of-mice-immunized-with-a-polyvalent-virosome.pdf
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Marc: |
LEADER 03009naa a2200433 a 4500
001 2156179
005 2023-09-04
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1186/s12985-023-02158-0$2DOI
100 1 $aFONSECA, F. M. da
245 $aImmunological profile of mice immunized with a polyvalent virosome-based influenza vaccine.$h[electronic resource]
260 $c2023
500 $aNa publicação: Francisco Noé Fonseca; Ana Paula Bastos.
520 $aAbstract Background: Influenza A virus (IAV) causes respiratory disease in pigs and is a major concern for public health. Vaccination of pigs is the most successful measure to mitigate the impact of the disease in the herds. Influenza-based virosome is an effective immunomodulating carrier that replicates the natural antigen presentation pathway and has tolerability profile due to their purity and biocompatibility. Methods: This study aimed to develop a polyvalent virosome influenza vaccine containing the hemagglutinin and neuraminidase proteins derived from the swine IAVs (swIAVs) H1N1, H1N2 and H3N2 subtypes, and to investigate its effectiveness in mice as a potential vaccine for swine. Mice were immunized with two vaccine doses (1 and 15 days), intramuscularly and intranasally. At 21 days and eight months later after the second vaccine dose, mice were euthanized. The humoral and cellular immune responses in mice vaccinated intranasally or intramuscularly with a polyvalent influenza virosomal vaccine were investigated. Results: Only intramuscular vaccination induced high hemagglutination inhibition (HI) titers. Seroconversion and seroprotection (>4-fold rise in HI antibody titers, reaching a titer of ?1:40) were achieved in 80% of mice (intramuscularly vaccinated group) at 21 days after booster immunization. Virus-neutralizing antibody titers against IAV were detected at 8 months after vaccination, indicating long-lasting immunity. Overall, mice immunized with the virosome displayed greater ability for B, effector-T and memory-T cells from the spleen to respond to H1N1, H1N2 and H3N2 antigens. Conclusions: All findings showed an efficient immune response against IAVs in mice vaccinated with a polyvalent virosome-based influenza vaccine.
650 $aInfluenza A virus
650 $aVaccination
650 $aInfluenza Aviaria
650 $aVacina
650 $aVacinação
650 $aVírus
653 $aNanovaccine
653 $aNanovacina
653 $aNanovacinação
653 $aSeroprotection
653 $aSoro imunológico
653 $aSoro proteção
653 $aSoroproteção
653 $aVaccine
700 1 $aHAACH, V.
700 1 $aBELLAVER, F. V.
700 1 $aBOMBASSARO, G.
700 1 $aGAVA, D.
700 1 $aSILVA, L. P. da
700 1 $aBARON, L. F.
700 1 $aSIMONELLY, M.
700 1 $aCARVALHO, W. A.
700 1 $aSCHAEFER, R.
700 1 $aBASTOS, A. P. A.
773 $tVirology Journal$gv. 20, 2023. Article number 187.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
29/03/2016 |
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Data da última atualização: |
28/04/2016 |
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Tipo da produção científica: |
Folder/Folheto/Cartilha |
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Autoria: |
SAMPAIO, A. B.; VIEIRA, D. L. M.; CORDEIRO, A. O. de O.; AQUINO, F. de G.; SOUSA, A. de P.; ALBUQUERQUE, L. B. de; SCHMIDT, I. B.; RIBEIRO, J. F.; PELLIZZARO, K. F.; SOUSA, F. S. de; MOREIRA, A. G.; SANTOS, A. B. P. dos; REZENDE, G. M.; SILVA, R. R. P.; ALVES, M.; MOTTA, C. P.; OLIVEIRA, M. C.; CORTES, C. de A.; OGATA, R. |
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Afiliação: |
DANIEL LUIS MASCIA VIEIRA, CENARGEN; FABIANA DE GOIS AQUINO, CPAC; LIDIAMAR BARBOSA DE ALBUQUERQUE, CPAC; JOSE FELIPE RIBEIRO, CPAC. |
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Título: |
Guia de restauração do Cerrado: volume 1: semeadura direta. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Brasília, DF: Universidade de Brasília: Rede de Sementes do Cerrado, 2015. |
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Páginas: |
40 p. |
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Descrição Física: |
il. |
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Idioma: |
Português |
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Conteúdo: |
Escolhendo o método de restauração; Semeadura direta; Cercamento; Aceiramento; Escolha de espécies; Manejo de sementes; Semeadura direta - plantio em linhas; Semeadura direta - plantio em área total; Monitoramento; Resultado de experimentos de semeadura direta em área total. |
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Palavras-Chave: |
Manejo de sementes; Recomposição vegetal. |
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Thesagro: |
Cerrado; Recurso natural. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/141879/1/Restauracao-semeadura-direta-cerrado-PDF-WEB.pdf
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Marc: |
LEADER 01380nam a2200385 a 4500
001 2044072
005 2016-04-28
008 2015 bl uuuu u0uu1 u #d
100 1 $aSAMPAIO, A. B.
245 $aGuia de restauração do Cerrado$bvolume 1: semeadura direta.$h[electronic resource]
260 $aBrasília, DF: Universidade de Brasília: Rede de Sementes do Cerrado$c2015
300 $a40 p.$cil.
520 $aEscolhendo o método de restauração; Semeadura direta; Cercamento; Aceiramento; Escolha de espécies; Manejo de sementes; Semeadura direta - plantio em linhas; Semeadura direta - plantio em área total; Monitoramento; Resultado de experimentos de semeadura direta em área total.
650 $aCerrado
650 $aRecurso natural
653 $aManejo de sementes
653 $aRecomposição vegetal
700 1 $aVIEIRA, D. L. M.
700 1 $aCORDEIRO, A. O. de O.
700 1 $aAQUINO, F. de G.
700 1 $aSOUSA, A. de P.
700 1 $aALBUQUERQUE, L. B. de
700 1 $aSCHMIDT, I. B.
700 1 $aRIBEIRO, J. F.
700 1 $aPELLIZZARO, K. F.
700 1 $aSOUSA, F. S. de
700 1 $aMOREIRA, A. G.
700 1 $aSANTOS, A. B. P. dos
700 1 $aREZENDE, G. M.
700 1 $aSILVA, R. R. P.
700 1 $aALVES, M.
700 1 $aMOTTA, C. P.
700 1 $aOLIVEIRA, M. C.
700 1 $aCORTES, C. de A.
700 1 $aOGATA, R.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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