| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Gado de Corte. |
|
Data corrente: |
02/01/2014 |
|
Data da última atualização: |
22/01/2020 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
CINTRA, L. C.; ZERLOTINI, A.; LOBO, F. P.; SILVA, F. R. da; GIACHETTO, P. F.; KUSER-FALCÃO, P. R.; SILVA, L. O. C. da; EGITO, A. A. do; SIQUEIRA, F.; SILVA, N. M. A. da; PAIVA, S. R.; YAMAGISHI, M. E. B.; CAETANO, A. R. |
|
Afiliação: |
LEANDRO CARRIJO CINTRA, CNPTIA; ADHEMAR ZERLOTINI NETO, CNPTIA; FRANCISCO PEREIRA LOBO, CNPTIA; FELIPE RODRIGUES DA SILVA, CNPTIA; POLIANA FERNANDA GIACHETTO, CNPTIA; PAULA REGINA KUSER FALCAO, CNPTIA; LUIZ OTAVIO CAMPOS DA SILVA, CNPGC; ANDREA ALVES DO EGITO, CNPGC; FABIANE SIQUEIRA, CNPGC; NAIARA MILAGRES AUGUSTO DA SILVA, CENARGEN; SAMUEL REZENDE PAIVA, Cenargen; MICHEL EDUARDO BELEZA YAMAGISHI, CNPTIA; ALEXANDRE RODRIGUES CAETANO, CENARGEN. |
|
Título: |
Sequencing and de novo genome assembly of a nelore (Bos indicus) bull. |
|
Ano de publicação: |
2013 |
|
Fonte/Imprenta: |
In: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. [Abstracts...]. [S.l.: s.n.], 2013. |
|
Páginas: |
Não paginado. |
|
Idioma: |
Inglês
Português |
|
Notas: |
Pôster 0522. |
|
Conteúdo: |
Bos indicus cattle breeds have been extensively used for dairy and beef production in tropical climates and present several natural adaptations to biotic and abiotic stresses found in these regions of the world. As breeder associations stride towards incorporating genomic tools into ongoing genetic evaluations and breeding programs to improve productivity and beef and milk quality traits, a (B. indicus) genome assembly represents an essential tool which will be vital to help identify and understand the underlying genetic variations that distinguish taurine and indicine cattle, which have diverged >250,000 years ago. DNA obtained from semen from a Nelore bull born in 1987, with an estimated cumulative inbreeding coefficient of 29.4%, and that can be traced to animals imported from India, was used to produce 100bp paired-end sequences from short (300 and 700bp) and long insert (3, 5 and 10 kbp) libraries, with an Illumina HiSeq platform. A total of 120Gbp were sequenced, corresponding to 45x raw coverage of the genome. The SOAP de novo assembler was used to build contigs and scaffolding. Several parameters sets were evaluated to obtain the best assembly based on the number of scaffolds, number of bases in scaffolds, N50, and total gap length. The best assembly obtained so far contains 2.7Gbp, 15,103 scaffolds with N50 of 649Kbp, and 756Mbp of gaps. Current results are being used to target additional sequencing of specific libraries to improve scaffold assembly. In addition, different sequencing technologies are also under evaluation for generating additional data to improve sequence assembly quality before comparisons with the reference (B. taurus) sequence are performed. MenosBos indicus cattle breeds have been extensively used for dairy and beef production in tropical climates and present several natural adaptations to biotic and abiotic stresses found in these regions of the world. As breeder associations stride towards incorporating genomic tools into ongoing genetic evaluations and breeding programs to improve productivity and beef and milk quality traits, a (B. indicus) genome assembly represents an essential tool which will be vital to help identify and understand the underlying genetic variations that distinguish taurine and indicine cattle, which have diverged >250,000 years ago. DNA obtained from semen from a Nelore bull born in 1987, with an estimated cumulative inbreeding coefficient of 29.4%, and that can be traced to animals imported from India, was used to produce 100bp paired-end sequences from short (300 and 700bp) and long insert (3, 5 and 10 kbp) libraries, with an Illumina HiSeq platform. A total of 120Gbp were sequenced, corresponding to 45x raw coverage of the genome. The SOAP de novo assembler was used to build contigs and scaffolding. Several parameters sets were evaluated to obtain the best assembly based on the number of scaffolds, number of bases in scaffolds, N50, and total gap length. The best assembly obtained so far contains 2.7Gbp, 15,103 scaffolds with N50 of 649Kbp, and 756Mbp of gaps. Current results are being used to target additional sequencing of specific libraries to improve scaffold assembly. In addition, di... Mostrar Tudo |
|
Palavras-Chave: |
Bioinformática; Sequenciamento de genoma. |
|
Thesagro: |
Bos indicus. |
|
Thesaurus Nal: |
bioinformatics; genome assembly; Nellore. |
|
Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/94595/1/P0522.odt
|
|
Marc: |
LEADER 02729nam a2200349 a 4500
001 1974758
005 2020-01-22
008 2013 bl uuuu u00u1 u #d
100 1 $aCINTRA, L. C.
245 $aSequencing and de novo genome assembly of a nelore (Bos indicus) bull.$h[electronic resource]
260 $aIn: INTERNATIONAL PLANT & ANIMAL GENOME, 21., 2013, San Diego. [Abstracts...]. [S.l.: s.n.]$c2013
300 $aNão paginado.
500 $aPôster 0522.
520 $aBos indicus cattle breeds have been extensively used for dairy and beef production in tropical climates and present several natural adaptations to biotic and abiotic stresses found in these regions of the world. As breeder associations stride towards incorporating genomic tools into ongoing genetic evaluations and breeding programs to improve productivity and beef and milk quality traits, a (B. indicus) genome assembly represents an essential tool which will be vital to help identify and understand the underlying genetic variations that distinguish taurine and indicine cattle, which have diverged >250,000 years ago. DNA obtained from semen from a Nelore bull born in 1987, with an estimated cumulative inbreeding coefficient of 29.4%, and that can be traced to animals imported from India, was used to produce 100bp paired-end sequences from short (300 and 700bp) and long insert (3, 5 and 10 kbp) libraries, with an Illumina HiSeq platform. A total of 120Gbp were sequenced, corresponding to 45x raw coverage of the genome. The SOAP de novo assembler was used to build contigs and scaffolding. Several parameters sets were evaluated to obtain the best assembly based on the number of scaffolds, number of bases in scaffolds, N50, and total gap length. The best assembly obtained so far contains 2.7Gbp, 15,103 scaffolds with N50 of 649Kbp, and 756Mbp of gaps. Current results are being used to target additional sequencing of specific libraries to improve scaffold assembly. In addition, different sequencing technologies are also under evaluation for generating additional data to improve sequence assembly quality before comparisons with the reference (B. taurus) sequence are performed.
650 $abioinformatics
650 $agenome assembly
650 $aNellore
650 $aBos indicus
653 $aBioinformática
653 $aSequenciamento de genoma
700 1 $aZERLOTINI, A.
700 1 $aLOBO, F. P.
700 1 $aSILVA, F. R. da
700 1 $aGIACHETTO, P. F.
700 1 $aKUSER-FALCÃO, P. R.
700 1 $aSILVA, L. O. C. da
700 1 $aEGITO, A. A. do
700 1 $aSIQUEIRA, F.
700 1 $aSILVA, N. M. A. da
700 1 $aPAIVA, S. R.
700 1 $aYAMAGISHI, M. E. B.
700 1 $aCAETANO, A. R.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Trigo. |
|
Data corrente: |
28/07/2015 |
|
Data da última atualização: |
28/03/2016 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
GUARIENTI, E. M.; MIRANDA, M. Z. de; ALBRECHT, J. C.; MORESCO, E. R.; SOARES SOBRINHO, J.; CHAGAS, J. H.; ITO, M. A.; SÓ e SILVA, M. |
|
Afiliação: |
ELIANA MARIA GUARIENTI, CNPT; MARTHA ZAVARIZ DE MIRANDA, CNPT; JULIO CESAR ALBRECHT, CPAC; EDINA REGINA MORESCO, CNPT; JOAQUIM SOARES SOBRINHO, CNPT; JORGE HENRIQUE CHAGAS, CNPT; MARCIO AKIRA ITO, CNPT; MÁRCIO SÓ E SILVA, CNPT. |
|
Título: |
Classificação comercial indicativa das cultivares de trigo da Embrapa - região homogênea de adaptação 4, safra 2015. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 8.; SEMINÁRIO TÉCNICO DO TRIGO, 9., 2014, Canela; REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 9.; SEMINÁRIO TÉCNICO DO TRIGO, 10., 2015, Passo Fundo. Anais... Passo Fundo: Biotrigo Genética: Embrapa Trigo, 2015. |
|
Descrição Física: |
2014 - Melhoramento, Aptidão industrial e Sementes - Trabalho 44. 1 CD-ROM. |
|
Idioma: |
Português |
|
Thesaurus NAL: |
wheat. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/127088/1/2014melhoramentotrabalho44.pdf
|
|
Marc: |
LEADER 00980nam a2200205 a 4500
001 2020703
005 2016-03-28
008 2015 bl uuuu u00u1 u #d
100 1 $aGUARIENTI, E. M.
245 $aClassificação comercial indicativa das cultivares de trigo da Embrapa - região homogênea de adaptação 4, safra 2015.$h[electronic resource]
260 $aIn: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 8.; SEMINÁRIO TÉCNICO DO TRIGO, 9., 2014, Canela; REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 9.; SEMINÁRIO TÉCNICO DO TRIGO, 10., 2015, Passo Fundo. Anais... Passo Fundo: Biotrigo Genética: Embrapa Trigo$c2015
300 $c2014 - Melhoramento, Aptidão industrial e Sementes - Trabalho 44. 1 CD-ROM.
650 $awheat
700 1 $aMIRANDA, M. Z. de
700 1 $aALBRECHT, J. C.
700 1 $aMORESCO, E. R.
700 1 $aSOARES SOBRINHO, J.
700 1 $aCHAGAS, J. H.
700 1 $aITO, M. A.
700 1 $aSÓ e SILVA, M.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Trigo (CNPT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
