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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
13/12/2023 |
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Data da última atualização: |
18/12/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CRUZ, T. I. da; ROCHA, D. C.; LANNA, A. C.; DEDICOVA, B.; VIANELLO, R. P.; BRONDANI, C. |
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Afiliação: |
THAÍS IGNEZ DA CRUZ, UFG; DHIÔVANNA CORRÊIA ROCHA, IAC; ANNA CRISTINA LANNA, CNPAF; BEATA DEDICOVA, SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES; ROSANA PEREIRA VIANELLO, CNPAF; CLAUDIO BRONDANI, CNPAF. |
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Título: |
Calcium-dependent protein kinase 5 (OsCPK5) overexpression in upland rice (Oryza sativa L.) under water deficit. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Plants, v. 12, n. 22, 3826, Nov. 2023. |
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ISSN: |
2223-7747 |
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DOI: |
https://doi.org/10.3390/plants12223826 |
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Idioma: |
Inglês |
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Conteúdo: |
Water deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plants under water deficit exhibited more leaf senescence, while the OsCPK5-overexpressing plants retained their green leaves. Overall, OsCPK5 overexpression induced diverse drought tolerance mechanisms, indicating the potential for future development of more drought-tolerant rice cultivars. MenosWater deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plant... Mostrar Tudo |
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Thesagro: |
Arroz; Deficiência Hídrica; Oryza Sativa; Resistência a Seca. |
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Thesaurus Nal: |
Drought; Gene expression; Rice; Senescence (aging); Water use efficiency. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1159651/1/plants-2023.pdf
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Marc: |
LEADER 02692naa a2200313 a 4500
001 2159651
005 2023-12-18
008 2023 bl uuuu u00u1 u #d
022 $a2223-7747
024 7 $ahttps://doi.org/10.3390/plants12223826$2DOI
100 1 $aCRUZ, T. I. da
245 $aCalcium-dependent protein kinase 5 (OsCPK5) overexpression in upland rice (Oryza sativa L.) under water deficit.$h[electronic resource]
260 $c2023
520 $aWater deficit significantly affects global crop growth and productivity, particularly in water-limited environments, such as upland rice cultivation, reducing grain yield. Plants activate various defense mechanisms during water deficit, involving numerous genes and complex metabolic pathways. Exploring homologous genes that are linked to enhanced drought tolerance through the use of genomic data from model organisms can aid in the functional validation of target species. We evaluated the upland rice OsCPK5 gene, an A. thaliana AtCPK6 homolog, by overexpressing it in the BRSMG Curinga cultivar. Transformants were assessed using a semi-automated phenotyping platform under two irrigation conditions: regular watering, and water deficit applied 79 days after seeding, lasting 14 days, followed by irrigation at 80% field capacity. The physiological data and leaf samples were collected at reproductive stages R3, R6, and R8. The genetically modified (GM) plants consistently exhibited higher OsCPK5 gene expression levels across stages, peaking during grain filling, and displayed reduced stomatal conductance and photosynthetic rate and increased water-use efficiency compared to non-GM (NGM) plants under drought. The GM plants also exhibited a higher filled grain percentage under both irrigation conditions. Their drought susceptibility index was 0.9 times lower than that of NGM plants, and they maintained a higher chlorophyll a/b index, indicating sustained photosynthesis. The NGM plants under water deficit exhibited more leaf senescence, while the OsCPK5-overexpressing plants retained their green leaves. Overall, OsCPK5 overexpression induced diverse drought tolerance mechanisms, indicating the potential for future development of more drought-tolerant rice cultivars.
650 $aDrought
650 $aGene expression
650 $aRice
650 $aSenescence (aging)
650 $aWater use efficiency
650 $aArroz
650 $aDeficiência Hídrica
650 $aOryza Sativa
650 $aResistência a Seca
700 1 $aROCHA, D. C.
700 1 $aLANNA, A. C.
700 1 $aDEDICOVA, B.
700 1 $aVIANELLO, R. P.
700 1 $aBRONDANI, C.
773 $tPlants$gv. 12, n. 22, 3826, Nov. 2023.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
24/04/2015 |
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Data da última atualização: |
24/04/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MASSUQUETO, A.; TEIXEIRA NETTO, M. V.; DASSI, S. C.; LIMA, L. B.; NATEL, J. C. C.; SUREK, D.; KRABBE, E. L. |
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Afiliação: |
UFPR; UFPR; UFPR; UFPR; LEPNAN; DIEGO SUREK, CNPSA; EVERTON LUIS KRABBE, CNPSA. |
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Título: |
Digestibilidade de dietas com diferentes formas físicas para frangos de corte. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: CONFERÊNCIA FACTA, 2014, Atibaia. [Anais...] Campinas: Fundação APINCO de Ciência e Tecnologia Avícolas, 2014. 1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
A peletização é o processamento térmico mais utilizado na indústria avícola, pois favorece o aproveitamento dos ingredientes e modifica a forma física da dieta. A pressão, umidade e temperatura empregadas por determinado tempo no processo, promovem alterações nas estruturas dos carboidratos, bem como das proteínas. O objetivo desse estudo foi avaliar o efeito de diferentes formas físicas (farelada ou peletizada/triturada com diferentes tempos de condicionamento) sobre o coeficiente de digestibilidade ileal aparente da matéria seca (CDIAMS), proteína bruta (CDIAPB) e energia digestível ileal (EDI) em frangos de corte de 1 a 25 dias. |
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Thesagro: |
Dieta; Frango de corte; Nutrição; Peletização. |
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Thesaurus NAL: |
Animal nutrition; Broiler chickens; Pelleted feeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122711/1/final7685.pdf
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Marc: |
LEADER 01486nam a2200265 a 4500
001 2014107
005 2015-04-24
008 2014 bl uuuu u00u1 u #d
100 1 $aMASSUQUETO, A.
245 $aDigestibilidade de dietas com diferentes formas físicas para frangos de corte.$h[electronic resource]
260 $aIn: CONFERÊNCIA FACTA, 2014, Atibaia. [Anais...] Campinas: Fundação APINCO de Ciência e Tecnologia Avícolas, 2014. 1 CD-ROM.$c2014
520 $aA peletização é o processamento térmico mais utilizado na indústria avícola, pois favorece o aproveitamento dos ingredientes e modifica a forma física da dieta. A pressão, umidade e temperatura empregadas por determinado tempo no processo, promovem alterações nas estruturas dos carboidratos, bem como das proteínas. O objetivo desse estudo foi avaliar o efeito de diferentes formas físicas (farelada ou peletizada/triturada com diferentes tempos de condicionamento) sobre o coeficiente de digestibilidade ileal aparente da matéria seca (CDIAMS), proteína bruta (CDIAPB) e energia digestível ileal (EDI) em frangos de corte de 1 a 25 dias.
650 $aAnimal nutrition
650 $aBroiler chickens
650 $aPelleted feeds
650 $aDieta
650 $aFrango de corte
650 $aNutrição
650 $aPeletização
700 1 $aTEIXEIRA NETTO, M. V.
700 1 $aDASSI, S. C.
700 1 $aLIMA, L. B.
700 1 $aNATEL, J. C. C.
700 1 $aSUREK, D.
700 1 $aKRABBE, E. L.
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Embrapa Suínos e Aves (CNPSA) |
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